CD18 Rat anti-Mouse, Brilliant Violet 605, Clone: M18/2, BD Optibuild™

Description

• The M18/2 antibody specifically recognizes the common β 2 chain of LFA-1 (CD11a/CD18, α L β 2 integrin), Mac-1 (CD11b/CD18, α M β 2 integrin), and gp150, 95 (CD11c/CD18, α X β 2 integrin)
• Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with the three integrin α chains (CD11a, CD11b, and CD11c)
• Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells
• The β 2 integrins are important mediators of leukocyte-endothelium interactions
• It has been reported that M18/2 antibody blocks in vivo /metastasis of the LB lymphoma to the spleen and that it blocks in vitro /formation of aggregates of LB cells and splenocytes
• However, other reports indicate that mAb M18/2 has no effect on CTLmediated killing, adherence of C3bi-sensitized erythrocytes to Mac-1, antigen-specific binding of T cells to antigen-producing cells, or rejection of cardiac allografts
• Recent in vitro /studies indicate that M18/2 antibody stimulates adhesion of Mac-1 to its ligands C3bi and ICAM-1, and it stimulates adhesion of LFA-1 to ICAM-1, but it has no effect upon the interactions of LFA-1 with ICAM-2 nor ICAM-3
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.