CD61 Mouse Anti-Rat, Brilliant Violet 605, Clone: F11, BD Optibuild™

Description

• The F11 monoclonal antibody specifically recognizes integrin 3 chain (CD61), which associates with the integrin v chain (CD51), to form the vitronectin receptor found on endothelial cells, myeloid cells, and osteoclasts, and with the IIb chain (CD41) on platelets and megakaryocytes
• Both receptors mediate adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor
• The F11 antibody reportedly blocks the in vitro attachment of osteoclasts to several ligands
• Weak F11 antibody reactivity with human osteoclasts, megakaryocytes, and platelets has also been reported
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.