CD61 Mouse Anti-Rat, Brilliant Violet 650, Clone: F11, BD Optibuild™

Description

• The F11 monoclonal antibody specifically recognizes integrin 3 chain (CD61), which associates with the integrin v chain (CD51), to form the vitronectin receptor found on endothelial cells, myeloid cells, and osteoclasts, and with the IIb chain (CD41) on platelets and megakaryocytes
• Both receptors mediate adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor
• The F11 antibody reportedly blocks the in vitro attachment of osteoclasts to several ligands
• Weak F11 antibody reactivity with human osteoclasts, megakaryocytes, and platelets has also been reported
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.