Vδ 6.3/2 TCR Hamster anti-Mouse, Brilliant Violet 510, Clone: 8F4H7B7, BD Optibuild™

Description

• The 8F4H7B7 antibody reacts with Vδ6.3/2 T-cell Receptor (TCR)-bearing T lymphocytes
• Originally defined as a member of the Vδ6 TCR subfamily, it is now proposed that the C57BL-derived Vδ6.3 is an allelic variant of Vδ6.2, found in A/J, AKR, BALB/c, C3H/He, and FVB mice
• mAb 8F4H7B7 crossreacts with Vδ6.4 and possibly Vδ6.6 in DBA/2 mice, and it also detects a subset ofγδTCR-bearing cells in CBA/J and C57L mice
• It does not recognize Vδ4, Vδ5, Vδ6.1, or Vδ6.5 TCR
• A subpopulation of thymocytes expressing Vδ6.3 or Vδ6.4 TCR (in C57BL/6 or DBA/2 mice, respectively) and low levels of CD90.2 (Thy-1.2) shares functional and phenotypic characteristics with NK-T cells
• SimilarδV 6.4 TCRexpressing lymphocytes make up significant proportions of theγδT-cell populations in the liver and spleen of DBA/2 mice
• Furthermore, T lymphocytes bearing Vδ6.3/2 TCR are found in the skin and intestinal epithelium and may represent a unique T-cell subpopulation with a potential for autoimmune reactivity
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set
• BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser
• BV510 is a polymer-based dye that is brighter than BD Horizon V500
• Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.