CD29 Mouse anti-Human, Brilliant Violet 421, Clone: MAR4., BD Optibuild™

Description

• The MAR4 monoclonal antibody specifically binds to CD29
• CD29 is a 130 kDa integrin 1 subunit that is expressed as a heterodimeric complex with one of six distinct subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors
• The 1 subunit has a broad tissue distribution; it is expressed on lymphocytes, monocytes and weakly on granulocytes, but not on erythrocytes
• These receptors are involved in a variety of cell-cell and cell-matrix interactions
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.