CD148 Hamster anti-Mouse, Brilliant Violet 421, Clone: 8A-1, BD Optibuild™

Description

• The 8A-1 monoclonal antibody specifically binds to CD148, which is also known as Protein tyrosine phosphatase receptor type J (Ptprj), Receptor-type tyrosine-protein phosphatase eta (R-PTP-eta, PTPη ), or Density-enhanced phosphatase-1 (DEP-1)
• CD148 is a receptor-like tyrosine phosphatase that plays important roles in regulating cell growth, proliferation, differentiation, adhesion, and migration
• It is expressed on a wide variety of cell types including epithelial cells, endothelial cells, and fibroblasts
• For cells of hemopoietic origin, it is variably expressed on macrophages, neutrophils, dendritic cells, and on developing and mature B cells
• CD148 is expressed on platelets and can regulate platelet activation
• Although expressed at low levels on thymocytes and mature T cells, its expression is upregulated upon T cell receptor (TCR)-mediated stimulation and may play a role in regulating TCR signaling
• CD148, along with CD45, are critical protein tyrosine phosphatases for regulating Src family kinase signaling networks in immune cells
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.