CD148 Hamster anti-Mouse, BUV395, Clone: 8A-1, BD Optibuild™

Description

• The 8A-1 monoclonal antibody specifically binds to CD148, which is also known as Protein tyrosine phosphatase receptor type J (Ptprj), Receptor-type tyrosine-protein phosphatase eta (R-PTP-eta, PTPη ), or Density-enhanced phosphatase-1 (DEP-1)
• CD148 is a receptor-like tyrosine phosphatase that plays important roles in regulating cell growth, proliferation, differentiation, adhesion, and migration
• It is expressed on a wide variety of cell types including epithelial cells, endothelial cells, and fibroblasts
• For cells of hemopoietic origin, it is variably expressed on macrophages, neutrophils, dendritic cells, and on developing and mature B cells
• CD148 is expressed on platelets and can regulate platelet activation
• Although expressed at low levels on thymocytes and mature T cells, its expression is upregulated upon T cell receptor (TCR)-mediated stimulation and may play a role in regulating TCR signaling
• CD148, along with CD45, are critical protein tyrosine phosphatases for regulating Src family kinase signaling networks in immune cells
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.