MAdCAM-1 Rat anti-Mouse, Brilliant Violet 510, Clone: MECA-89, BD Optibuild™

Description

• The MECA-89 antibody specifically recognizes the mucosal vascular addressin cell adhesion molecule (MAdCAM-1)
• In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes
• In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria; it is also expressed on sinus-lining cells in the spleen
• MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice and is involved in the development of diabetes and insulitis
• Furthermore, there is evidence that IFN-γcan induce MAdCAM-1 expression in non-mucosal sites in adult mice
• MAdCAM-1 is a predominant ligand for integrinα4β7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin)
• MECA-89 mAb binds to the second domain of MAdCAM-1 and does not block MAdCAM-1-dependent binding in vitro /
• Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set
• BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser
• BV510 is a polymer-based dye that is brighter than BD Horizon V500
• Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.