CD104 Rat anti-Human, Brilliant Violet 421, Clone: 439-9B, BD Optibuild™

Description

• The 439-9B monoclonal antibody specifically recognizes CD104, integrinβ4 chain, a 205 kDa transmembrane glycoprotein, which associates with CD49f (integrinα6 chain) to form theα6/β4 (CD49f/CD104) complex
• It is expressed on epithelial cells, Schwann cells, and some tumor cells
• The CD49f/CD104 complex is located in the hemidesmosomes of the epidermis, suggesting its role in epidermal cell-basement membrane adhesion
• The clone 439-9B was clustered as CD104 at the fifth Human Leucocyte Differentiation Antigen International Workshop
• It may be used for immunoprecipitation, immunoblotting and immunohistochemistry on frozen tissue sections
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.