CD182 Mouse anti-Human, Brilliant Violet 421, Clone: 6C6, BD Optibuild™

Description

• The 6C6 monoclonal antibody specifically binds to CD182
• CD182 is also known as the interleukin-8 receptor B (IL-8RB), a G-protein-linked seven transmembrane domain protein, expressed on monocytes and granulocytes
• It is also expressed on a subset of T cells
• Both IL-8RA (CXCR1) and IL-8RB (CXCR2) are high affinity receptors for IL-8
• IL-8 is an inflammatory cytokine which induces chemotaxis of neutrophils, basophils and T lymphocytes
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.