CD182 Mouse anti-Human, Brilliant Violet 711, Clone: 6C6, BD Optibuild™

Description

• The 6C6 monoclonal antibody specifically binds to CD182
• CD182 is also known as the interleukin-8 receptor B (IL-8RB), a G-protein-linked seven transmembrane domain protein, expressed on monocytes and granulocytes
• It is also expressed on a subset of T cells
• Both IL-8RA (CXCR1) and IL-8RB (CXCR2) are high affinity receptors for IL-8
• IL-8 is an inflammatory cytokine which induces chemotaxis of neutrophils, basophils and T lymphocytes
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.