CD32 Mouse anti-Human, Brilliant Violet 510, Clone: 3D3, BD Optibuild™

Description

• The 3D3 monoclonal antibody specifically recognizes FcγRII (CD32), a 40 kDa, polymorphic type I transmembrane glycoprotein that serves as a low affinity receptor for aggregated IgG
• This highly glycosylated molecule (encoded by at least two different genes) is expressed on monocytes, granulocytes, platelets and B cells
• Unlike the FLI28.26 mAb, the 3D3 mAb detected a polymorphic CD32 antigen expressed on B cells of all donors, but only on platelets, monocytes and granulocytes of some donors
• The platelets from 3D3+ donors respond to certain stimulatory mAb such as CD165 (clone SN2) which results in aggregation
• On the other hand, the platelets from 3D3 negative donors do not form aggregates after stimulation
• Individuals can be divided into two groups as responder and non-responder depending on expression, or non-expression, of 3D3
• In comparison to the 3D3 mAb, the FLI8.26 mAb detects a monomorphic CD32 antigen expressed on all human donors
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set
• BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser
• BV510 is a polymer-based dye that is brighter than BD Horizon V500
• Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.