IgE Mouse anti-Human, Brilliant Violet 650, Clone: G7-26, BD Optibuild™

Description

• The G7-26 monoclonal antibody specifically recognizes the constant region of human Immunoglobulin E (IgE)
• It does not crossreact with other immunoglobulin heavy chain isotypes
• IgE exists in a transmembrane form that is expressed by B lymphocytes and serves as an antigen receptor
• Soluble IgE is produced and secreted by activated B cells and plasma cells
• IgE may bind through its constant region to cell surface receptors, such as the high-affinity ( ) or low-affinity ( /CD23) receptors for IgE
• Fc RI is expressed on mast cells, basophils, and at lower levels, on dendritic cells and monocytes
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.