Ly-6A/E Rat anti-Mouse, Brilliant Violet 605, Clone: E13-161.7, BD Optibuild™

Description

• The E13-161.7 mAb recognizes Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family
• Ly-6A/E is also known as stem cell antigen 1 (Sca-1/Sca1), or T-cell-activating protein (TAP)
• Ly-6A/E is a phosphatidylinositol-anchored protein of ∽18kDa that is expressed on multipotent hematopoietic stem cells (HSC) in mice with both Ly-6 haplotypes
• Sca-1+ HSC are found in the adult bone marrow and fetal liver, but not in the early embryo yolk sac or intraembryonic hematopoietic sites, and can be mobilized to the peripheral blood and spleen in the adult
• In mice expressing the Ly-6.2 haplotype, Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes, myeloid cells, and thymic and peripheral T lymphocytes, on the earliest intrathymic T-cell precursor population, and in several non-hematopoietic tissues
• Strains with the Ly-6.1 haplotype have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of T cells from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen
• Studies with the D7 antibody have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation
• Purified E13-161.7 mAb can block binding of FITC-conjugated D7 antibody (anti-Ly-6A/E) to mouse splenocytes, but purified mAb D7 is unable to block binding of FITC-conjugated E13-161.7 antibody
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.