Ly-6A/E Rat anti-Mouse, BUV395, Clone: E13-161.7, BD Optibuild™

Description

• The E13-161.7 mAb recognizes Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family
• Ly-6A/E is also known as stem cell antigen 1 (Sca-1/Sca1), or T-cell-activating protein (TAP)
• Ly-6A/E is a phosphatidylinositol-anchored protein of ∽18kDa that is expressed on multipotent hematopoietic stem cells (HSC) in mice with both Ly-6 haplotypes
• Sca-1+ HSC are found in the adult bone marrow and fetal liver, but not in the early embryo yolk sac or intraembryonic hematopoietic sites, and can be mobilized to the peripheral blood and spleen in the adult
• In mice expressing the Ly-6.2 haplotype, Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes, myeloid cells, and thymic and peripheral T lymphocytes, on the earliest intrathymic T-cell precursor population, and in several non-hematopoietic tissues
• Strains with the Ly-6.1 haplotype have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of T cells from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen
• Studies with the D7 antibody have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation
• Purified E13-161.7 mAb can block binding of FITC-conjugated D7 antibody (anti-Ly-6A/E) to mouse splenocytes, but purified mAb D7 is unable to block binding of FITC-conjugated E13-161.7 antibody
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.