Qa-1(b) Mouse anti-Mouse, Brilliant Violet 510, Clone: 6A8.6F10.1A6, BD Optibuild™

Description

• The 6A8.6F10.1A6 monoclonal antibody reacts with the Qa-1[b] alloantigen, which is a nonclassical MHC class I (Class Ib) molecule encoded by the T23 /gene of the H-2 /complex
• Qa-1 associates withβ2-microglobulin and is expressed at low levels on most leukocytes and many other cell types
• Its level of cell-surface expression is upregulated by IFNγor specific peptides
• Qa-1 is an oligomorphic molecule which presents a limited pool of peptides to T lymphocytes bearingαβandγδTCR and binds to a large subpopulation of NK cells
• In fact, Qa-1[b] is the ligand for CD94/NKG2A, CD94/NKG2C, and CD94/NKG2E receptors, which are expressed on NK cells
• Furthermore, it has been reported that Qa-1 expressed on activated B lymphocytes is involved in immunoregulation by inducing T-cell-mediated suppression of antibody responses
• The 6A8.6F10.1A6 mAb can detect Qa-1[b] on activated splenocytes from C57BL/6 and BALB/c mice (both Qa-1[b]), but not from A/j mice (Qa-1[a]), and it can block target cell recognition by CTLs specific for Qa-1-presented antigen
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set
• BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser
• BV510 is a polymer-based dye that is brighter than BD Horizon V500
• Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.