CD11c Mouse anti-Human, Brilliant Violet 421, Clone: S-HCL-3, BD Optibuild™

Description

• The S-HCL-3 monoclonal antibody specifically binds to CD11c which is encoded by ITGAX (Integrin alpha X, complement component 3 receptor 4 subunit)
• CD11c is a ñ150 kDa type I transmembrane glycoprotein that is expressed on dendritic cells, monocytes, macrophages, granulocytes, NK cells and subsets of B and T cells
• CD11c is also present on hairy-cell leukemias and on some acute myeloid leukemias (AML)
• The S-HCL-3 antibody stains sinus histiocytes of lymph node, tonsil, and spleen; macrophages in the cortex and medulla of thymus; Kupffer cells in liver; elongated intertubular cells in kidney; and alveolar macrophages in lung tissue
• In malignant tissue, the antibody stains true histiocytic lymphomas; it does not stain carcinomas or T or B lymphomas
• CD11c associates with CD18 (ITGB2/Integrin beta-2) to form the CD11c/CD18 heterodimer which is likewise known as Leukocyte adhesion receptor p150,95 or Complement receptor type 4 (CR4)
• Ligands for this heterodimeric adhesion receptor include fibrinogen, iC3b, CD23, and CD54 (ICAM-1)
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.