CD49d Mouse anti-Human, BUV395, Clone: 9F10, BD Optibuild™

Description

• The 9F10 monoclonal antibody specifically reacts with the integrinα4 chain, that is expressed as a heterodimer with either of twoβintegrin subunits,β1 (CD29) orβ7
• Theα4β1 integrin (VLA-4) is expressed on lymphocytes, monocytes, thymocytes, NK cells, and several B- and T-cell lines, and mediates binding to VCAM-1 (CD106) and the CS-1 region of fibronectin
• Theα4β7 integrin has a similar tissue distribution, except it is found on only a small subpopulation of thymocytes
• Integrinα4β7 also binds fibronectin and VCAM-1, and has been shown in the mouse to preferentially bind the mucosal vascular addressin molecule, MAdCAM-1
• This antibody is useful for studies of the expression by and function of cells that expressα4 chain-containing integrins
• This clone cross-reacts with a subset of peripheral blood lymphocytes, monocytes, and some granulocytes of baboon and both rhesus and cynomolgus macaque monkeys
• The distribution on leukocytes is similar to that observed with human peripheral blood leukocytes
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.