CD33 Mouse anti-Human, BUV395, Clone: HIM3-4, BD Optibuild™

Description

• The HIM3-4 monoclonal antibody specifically binds to CD33 which is also known as Sialic acid-binding Ig-like lectin 3 (Siglec-3), gp67, or p67
• CD33 is a 67 kDa type I transmembrane glycoprotein expressed on monocytes, activated T cells, myeloid progenitors as well as mast cells
• CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells
• This glycoprotein can reportedly function as a sialic acid-dependent cell adhesion molecule
• This function can be modulated by endogenous sialoglycoconjugates when CD33 is expressed on the membrane
• HIM3-4 reacts with human and monkey granulocytes and monocytes
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.