PAPPA Monoclonal Antibody (004-09), Invitrogen™

Description

• BTE 004-09-02 detects Pregnancy-associated plasma protein A (PAPP-A) from human samples
• Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A
• BTE 004-09-02 has been successfully used in ELISA, Western blotting and Immunohistochemistry procedures
• In sandwich ELISA the optimal combination is BTE 004-09-02 used as coating antibody and biotinylated ABS 006-24-02 used as detection antibody
• BTE 004-09-02 can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat
• BTE 004-09-02 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of ABS 006-01-02 and ABS 006-24-02
• The epitope of BTE 004-09-02 is different from ABS 006-01-02 and ABS 006-24-02
• The epitope is located on the PAPP-A subunit of the heterotetramer
• NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterottetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women
• The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits
• Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down's syndrome (trisomy 21).