Collagen III (soluble) Monoclonal Antibody (1E7-D7/Col3), Invitrogen™

Description

• CSI 007-01-02 is highly specific for type III collagen
• It has been shown to have no cross-reactivity with type I and V collagens by ELISA and immunoblotting
• There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin)
• CSI 007-01-02 shows reactivity with human, dog, rat and kangaroo in type III collagens by ELISA and immunoblotting
• The antibody does not show reactivity with calf and sheep type III collagen by ELISA and immunoblotting
• CSI 007-01-02 has been successfully used in Western blotting, ELISA, and immunohistochemistry (frozen) procedures
• CSI 007-01 has been used successfully for immunohistology on frozen unfixed sections of human and kangaroo skin, and of new dog tissue associated with biomaterial implants
• If fixation of tissue is required, acetone or ethanol is recommended
• The specificity of the CSI 007-01-02 epitope is located approximately 100 nm from the N-terminal of the molecule
• NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen
• Mutations in th gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms
• Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.