GM-CSF Monoclonal Antibody (1089), Biotin, Invitrogen™

Description

• M501B targets Human GM-CSF in ELISA applications and shows reactivity with Human samples
• The M501B immunogen is glycosylated, CHO-derived recombinant human GM-CSF
• M501B detects Human GM-CSF which has a predicted molecular weight of approximately 14 kDa
• The M501B GMCSF antibody (biotinylated conjugate of clone 1089) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M500A (clone 3092)
• Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection
• Biotinylated antibody M501B (clone 1089) and antibody M500A (clone 3092) have successfully been used in combination with recombinant GMCSF protein SGMCSF in ELISA applications
• The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes
• The active form of the protein exists as a homodimer in the extracellular space
• This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia
• Other genes in the cluster include interleukins 4, 5, and 13
• The gene is involved in promoting tissue inflammation
• Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome
• Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.