GM-CSF Monoclonal Antibody (22E9), Invitrogen™

Description

• MM500C targets Mouse GM-CSF in ELISA, and WB applications and shows reactivity with mouse samples
• The MM500C immunogen is recombinant mouse GM-CSF
• MM500C detects Mouse GM-CSF which has a predicted molecular weight of approximately 14 kDa
• The MM500C mouse GMCSF antibody (clone 22E9) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM500DB (biotinylated conjugate of clone 31G6) Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection
• Antibody MM500C (clone 22E9) and biotinylated antibody MM500DB (clone 31G6) have successfully been used in combination with recombinant mouse GMCSF protein SMGMCSF in ELISA applications
• This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice
• The resulting ascites is collected from the mice and the antibody is purified
• This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg)
• The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes
• The active form of the protein exists as a homodimer in the extracellular space
• This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia
• Other genes in the cluster include interleukins 4, 5, and 13
• The gene is involved in promoting tissue inflammation
• Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome
• Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.