TNF alpha Polyclonal Antibody, Invitrogen™

Description

• PRTNFAI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Rat samples
• The PRTNFAI immunogen is recombinant rat TNF alpha
• PRTNFAI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa
• The PRTNFAI rat TNFa polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with polyclonal biotinylated detection antibody PRTNFAI
• Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection
• Endotoxin levels are less than or equal to 10 μg/mL
• TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily
• This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR
• TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation
• TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions
• Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice
• Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors
• TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity
• This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.