CD27 Monoclonal Antibody (O323), NovaFluor™ Blue 610-70S, eBioscience™, Invitrogen™

Description

• The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily
• CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation
• CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction
• The O323 antibody has been reported for use in flow cytometric analysis
• The O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 uL (0.8 ug) per test
• A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer
• Use this buffer whenever staining with NovaFluor conjugates, including single-color compensation controls using cells
• Whenever possible, we recommend adding CellBlox Blocking Buffer to antibody cocktails/master mixes prior to combining with cells
• Add 5 uL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended
• For single-color controls, use 5 uL of CellBlox Blocking Buffer per 100uL of cell sample containing 10^3 to 10^8 cells
• Excitation: 509 nm; Emission: 614 nm; Laser: 488 nm (Blue) Laser CD27 Monoclonal antibody specifically detects CD27 in Human samples
• It is validated for Flow Cytometry