E-cadherin Monoclonal Antibody (MB2), Invitrogen™

Description

• MA1-06300 detects E-cadherin in human samples
• MA1-06300 has sucessfully been used in Western blotting, Flow cytometry, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry
• By Western blotting, it detects a ∽120 kDa protein representing E-cadherin
• The MA1-06300 immunogen is MCF-7/AZ cells expressing E-cadherin at their cell surface
• Store at 4oC, or in small aliquots at -20oC
• E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily
• The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail
• Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer
• Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis
• The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization
• Identified transcript variants arise from mutation at consensus splice sites
• E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity
• In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16
• Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.