CDH11 Monoclonal Antibody (16A), Invitrogen™

Description

• MA1-06306 detects OB-cadherin in human and rat samples
• MA1-06306 has sucessfully been used in Western blotting, immunocytochemistry, and immunohistochemistry
• The MA1-06306 immunogen is affinity purified extracellular domain of human cadherin-11-GST fusion protein
• Store at 4oC, or in small aliquots at -20oC
• This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion
• All cadherins are single-pass transmembrane proteins with a variable number of 110 amino acid extracellular cadherin (EC) domains
• Classical cadherins contain five large N-terminal EC domains, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain
• Based on amino acid alignment, classical cadherins are divided into type I and type II subgroups
• Type I cadherins, including cadherins E (epithelial), N (neural), P (placental), and R (retinal) cadherin differ from type I cadherins in their specific amino acid sequences
• Type II cadherins include human cadherin-5, -6, -8, -11, and -12
• Type II cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins
• Cadherin-11 (OB-Cadherin) is a marker of the loosely connected and migratory cellular elements of the mesenchyme
• Strong expression of Cadherin-11 has been noted in brain, spinal cord, bone marrow and bone cells, and is regulated in human endometrial glandular epithelial and stromal cells
• Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance
• A variant Cadherin-11 gene (variant form) encodes a truncated protein with an unusual cytoplasmic domain not present in other cadherins
• The variant protein is generated by alternative splicing, utilizing a 179 bp intronic sequence
• Recently, Cadherin-11 mRNA and protein were found to be expressed in most invasive breast cancer cell lines tested but not in non-invasive cell lines, suggesting that Cadherin-11 may be a marker for aggressive, invasive tumor subsets.