TGF beta-1 Monoclonal Antibody (TB21), Invitrogen™

Description

• Recommended positive controls: Ovine ovarian tissue, breast cancer sections
• By Western blot, this antibody detects detects dimeric (25 kDa) and monomeric (12.5 kDa) forms of TGF beta, under non-reducing and reducing conditions, respectively Store product as a concentrated solution
• Centrifuge briefly prior to opening the vial
• TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues
• Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain
• Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins
• Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP
• TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types
• Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors
• TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer
• The mature peptide may also form heterodimers with other TGF beta family members
• The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.