Mu-Calpain Monoclonal Antibody (9A4H8D3), Invitrogen™

Description

• MA3-940 detects mu-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, mouse lung, pig cultured cells and hamster and rabbit samples
• This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin
• MA3-940 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunocytochemistry procedures
• By Western blot, this antibody detects an 80 kDa protein representing mu-calpain from human platelets and erythrocytes and HeLa and A431 cell lysates
• Immunocytochemical staining of mu-calpain in LLC-PK1 cells with MA3-940 results in diffuse cytoplasmic staining
• This product has not been shown to be effective in immunoprecipitation experiments
• The MA3-940 antigen is purified bovine skeletal muscle 80 kDa mu-calpain subunit
• This antibody recognizes an epitope between amino acids 465-520 (domain III) of human mu-calpain
• The calpains including Calpain 1 (CAPN1), calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases
• The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins
• The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit
• This gene encodes the large subunit of the ubiquitous enzyme, calpain 1
• Several transcript variants encoding two different isoforms have been found for this gene
• Diseases associated with CAPN1 include Spastic Paraplegia 76, Autosomal Recessive and Spasticity.