MABE1823100
AsCpf1, Mouse anti-Bacteria, Clone: 3D3-F7, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE1823100-Each-of-1 | In Stock | ₹ 43,067.48 |
MABE1823100 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 43,067.48
GST (18%): ₹ 7,752.146
Total Price: ₹ 50,819.626
Antigen
AsCpf1
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
Cas12a;Cpf1
Immunogen
His-tagged recombinant fragment corresponding to 197 amino acids from the C-terminal region of Acidaminococcus Cas12a.
Quantity
100 μg
Research Discipline
Epigenetics & Nuclear Function
Test Specificity
Clone 3D3-F7 specifically detects CRISPR-associated endonuclease Cas12a.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG2a κ
Applications
Western Blot
Clone
3D3-F7
Dilution
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AsCpf1 in HEK293 cells expressing HA-tagged AsCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Gene Alias
EC 3.1.27.2;AsCpf1;CRISPR-associated endonuclease Cpf1
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Bacteria
Form
Purified
Related Products
Description
- Anti-AsCpf1, clone 3D3-F7, Cat
- No
- MABE1823, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cpf1 and has been tested in Western Blotting
- CRISPR-associated endonuclease Cas12a (UniProt: U2UMQ6; also known as EC: 3.1.27.2, AsCpf1, CRISPR-associated endonuclease Cpf1) I encoded by the Cas12a (also known as cpf1) gene in Acidaminococcus species
- CRISPR is an adaptive immune system that provides protection against mobile genetic elements, such as viruses, transposable elements, and conjugative plasmids
- CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids
- They are transcribed and processed into CRISPR RNA (crRNA)
- Cas12a, an endonuclease from the type V-A CRISPR system, has several unique characteristics when compared to Cas9 system that increase the utility of CRISPR-based gene editing
- Cas12a mediated cleavage utilizes a single and short CRISPR RNA) without requiring trans-activating crRNA
- Cas12a recognize a 5' T-rich protospacer adjacent motif (PAM, TTTN for this specific enzyme), unlike Cas9 enzymes which recognize 3' G-rich PAMs, thus this enzyme increases the possibilities for genome editing
- The endonuclease activity of Cas12a results in staggered 4-base 5' overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively.
Compare Similar Items
Show Difference
Antigen: AsCpf1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: Cas12a;Cpf1
Immunogen: His-tagged recombinant fragment corresponding to 197 amino acids from the C-terminal region of Acidaminococcus Cas12a.
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: Clone 3D3-F7 specifically detects CRISPR-associated endonuclease Cas12a.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2a κ
Applications: Western Blot
Clone: 3D3-F7
Dilution: Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AsCpf1 in HEK293 cells expressing HA-tagged AsCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Gene Alias: EC 3.1.27.2;AsCpf1;CRISPR-associated endonuclease Cpf1
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Bacteria
Form: Purified
Antigen:
AsCpf1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
Cas12a;Cpf1
Immunogen:
His-tagged recombinant fragment corresponding to 197 amino acids from the C-terminal region of Acidaminococcus Cas12a.
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
Clone 3D3-F7 specifically detects CRISPR-associated endonuclease Cas12a.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2a κ
Applications:
Western Blot
Clone:
3D3-F7
Dilution:
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AsCpf1 in HEK293 cells expressing HA-tagged AsCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Gene Alias:
EC 3.1.27.2;AsCpf1;CRISPR-associated endonuclease Cpf1
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Bacteria
Form:
Purified
Antigen: AsCpf1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: Cas12a;Cpf1
Immunogen: His-tagged recombinant fragment corresponding to 197 amino acids from the C-terminal region of Acidaminococcus Cas12a.
Quantity: 25 μg
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: Clone 3D3-F7 specifically detects CRISPR-associated endonuclease Cas12a.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2a κ
Applications: Western Blot
Clone: 3D3-F7
Dilution: Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AsCpf1 in HEK293 cells expressing HA-tagged AsCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Gene Alias: EC 3.1.27.2;AsCpf1;CRISPR-associated endonuclease Cpf1
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Bacteria
Form: Purified
Antigen:
AsCpf1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
Cas12a;Cpf1
Immunogen:
His-tagged recombinant fragment corresponding to 197 amino acids from the C-terminal region of Acidaminococcus Cas12a.
Quantity:
25 μg
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
Clone 3D3-F7 specifically detects CRISPR-associated endonuclease Cas12a.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2a κ
Applications:
Western Blot
Clone:
3D3-F7
Dilution:
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AsCpf1 in HEK293 cells expressing HA-tagged AsCpf1 (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).
Gene Alias:
EC 3.1.27.2;AsCpf1;CRISPR-associated endonuclease Cpf1
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Bacteria
Form:
Purified
Antigen: beta-Catenin
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: CTNNB1;CTNNB;OK/sw-cl.35;PRO2286
Immunogen: BSA-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human beta-catenin 1.
Quantity: 100 μL
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: This rat monoclonal antibody detects beta-catenin 1 unmodified and methylated or acetylated on lysine 49. It targets an epitope with in 19 amino acids from the N-terminal region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Applications: ChIP Assay, Dot Blot, Immunoprecipitation, Western Blot
Clone: mAb-Cat1
Dilution: Western Blotting Analysis: A representative lot detected beta-Catenin in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Immunoprecipitation Analysis: A representative lot immunoprecipitated beta-Catenin in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Dot Blot Analysis: A representative lot detected beta-Catenin in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824). Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated beta-Catenin in Chromatin Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
Gene Alias: beta-Catenin 1;Catenin beta-1
Host Species: Rat
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
beta-Catenin
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
CTNNB1;CTNNB;OK/sw-cl.35;PRO2286
Immunogen:
BSA-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human beta-catenin 1.
Quantity:
100 μL
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
This rat monoclonal antibody detects beta-catenin 1 unmodified and methylated or acetylated on lysine 49. It targets an epitope with in 19 amino acids from the N-terminal region.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Applications:
ChIP Assay, Dot Blot, Immunoprecipitation, Western Blot
Clone:
mAb-Cat1
Dilution:
Western Blotting Analysis: A representative lot detected beta-Catenin in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Immunoprecipitation Analysis: A representative lot immunoprecipitated beta-Catenin in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Dot Blot Analysis: A representative lot detected beta-Catenin in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824). Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated beta-Catenin in Chromatin Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
Gene Alias:
beta-Catenin 1;Catenin beta-1
Host Species:
Rat
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Antigen: beta-Catenin
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: CTNNB1;CTNNB;OK/sw-cl.35;PRO2286
Immunogen: BSA-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human beta-catenin 1.
Quantity: 25 μL
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: This rat monoclonal antibody detects beta-catenin 1 unmodified and methylated or acetylated on lysine 49. It targets an epitope with in 19 amino acids from the N-terminal region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Applications: ChIP Assay, Dot Blot, Immunoprecipitation, Western Blot
Clone: mAb-Cat1
Dilution: Western Blotting Analysis: A representative lot detected beta-Catenin in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Immunoprecipitation Analysis: A representative lot immunoprecipitated beta-Catenin in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Dot Blot Analysis: A representative lot detected beta-Catenin in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824). Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated beta-Catenin in Chromatin Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
Gene Alias: beta-Catenin 1;Catenin beta-1
Host Species: Rat
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
beta-Catenin
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
CTNNB1;CTNNB;OK/sw-cl.35;PRO2286
Immunogen:
BSA-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human beta-catenin 1.
Quantity:
25 μL
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
This rat monoclonal antibody detects beta-catenin 1 unmodified and methylated or acetylated on lysine 49. It targets an epitope with in 19 amino acids from the N-terminal region.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Applications:
ChIP Assay, Dot Blot, Immunoprecipitation, Western Blot
Clone:
mAb-Cat1
Dilution:
Western Blotting Analysis: A representative lot detected beta-Catenin in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Immunoprecipitation Analysis: A representative lot immunoprecipitated beta-Catenin in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Dot Blot Analysis: A representative lot detected beta-Catenin in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824). Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated beta-Catenin in Chromatin Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
Gene Alias:
beta-Catenin 1;Catenin beta-1
Host Species:
Rat
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified