MABF2162100
HLA-F, Mouse anti-Human, Clone: 3D11, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABF2162100-Each-of-1 | In Stock | ₹ 41,308.37 |
MABF2162100 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 41,308.37
GST (18%): ₹ 7,435.507
Total Price: ₹ 48,743.877
Antigen
HLA-F
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
HLA-F;HLA-5.4;HLAF
Immunogen
Full-length human recombinant HLA-F Heavy chains (excluding the signal sequence).
Quantity
100 μg
Research Discipline
Inflammation & Immunology
Gene ID (Entrez)
NP_061823
Target Species
Human
Form
Purified
Applications
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Clone
3D11
Dilution
ELISA Analysis: A representative lot detected HLA-F in ELISA applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Western Blotting Analysis: A representative lot detected HLA-F in Western Blotting applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18; Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).Flow Cytometry Analysis: A representative lot detected HLA-F in Flow Cytometry applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Immunohistochemistry Analysis: A representative lot detected HLA-F in Immunohistochemistry applications (Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).
Gene Alias
HLA class I histocompatibility antigen alpha chain F;CDA12;HLA F antigen;Leukocyte antigen F;MHC class I antigen F
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 3D11 specifically detects human HLA-F. It targets an epitope with in the alpha 2 domain from the N-terminal half.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-HLA-F, clone 3D11, Cat
- No
- MABF2162, is a mouse monoclonal antibody that detects HLA class I histocompatibility antigen, alpha chain F and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting
- HLA class I histocompatibility antigen, alpha chain F (UniProt: P30511; also known as CDA12, HLA F antigen, Leukocyte antigen F, MHC class I antigen F) is encoded by the HLA-F (also known as HLA-5.4, HLAF) gene (Gene ID: 3134) in human
- HLA-F is a single-pass type I membrane heterodimeric protein that is involved in the presentation of foreign antigens to the immune system
- It is expressed in B cells, NK cells, monocytes, and T cells and its expression is reported to be coincident with the activated immune response
- It is mainly localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types
- It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off
- The mature form has an extracellular domain (aa 22-305), a transmembrane domain (aa 306-329), and a short cytoplasmic domain (aa 330-346)
- The HLA-F locus has very low allelic polymorphism and is highly conserved in distantly related non-human primates indicating its conserved function
- It is involved in the regulation of immune system in pregnancy, infection, and autoimmunity by signaling through NK cell receptors
- (Ref.: Dulberger, CL et al
- (2017)
- Immunity 46(6); 1018 1029; Lee, N., et al
- (2010)
- Eur
- J
- Immunol
- 40(8): 2308 2318).
Compare Similar Items
Show Difference
Antigen: HLA-F
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: HLA-F;HLA-5.4;HLAF
Immunogen: Full-length human recombinant HLA-F Heavy chains (excluding the signal sequence).
Quantity: 100 μg
Research Discipline: Inflammation & Immunology
Gene ID (Entrez): NP_061823
Target Species: Human
Form: Purified
Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Clone: 3D11
Dilution: ELISA Analysis: A representative lot detected HLA-F in ELISA applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Western Blotting Analysis: A representative lot detected HLA-F in Western Blotting applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18; Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).Flow Cytometry Analysis: A representative lot detected HLA-F in Flow Cytometry applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Immunohistochemistry Analysis: A representative lot detected HLA-F in Immunohistochemistry applications (Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).
Gene Alias: HLA class I histocompatibility antigen alpha chain F;CDA12;HLA F antigen;Leukocyte antigen F;MHC class I antigen F
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 3D11 specifically detects human HLA-F. It targets an epitope with in the alpha 2 domain from the N-terminal half.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
HLA-F
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
HLA-F;HLA-5.4;HLAF
Immunogen:
Full-length human recombinant HLA-F Heavy chains (excluding the signal sequence).
Quantity:
100 μg
Research Discipline:
Inflammation & Immunology
Gene ID (Entrez):
NP_061823
Target Species:
Human
Form:
Purified
Applications:
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Clone:
3D11
Dilution:
ELISA Analysis: A representative lot detected HLA-F in ELISA applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Western Blotting Analysis: A representative lot detected HLA-F in Western Blotting applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18; Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).Flow Cytometry Analysis: A representative lot detected HLA-F in Flow Cytometry applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Immunohistochemistry Analysis: A representative lot detected HLA-F in Immunohistochemistry applications (Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).
Gene Alias:
HLA class I histocompatibility antigen alpha chain F;CDA12;HLA F antigen;Leukocyte antigen F;MHC class I antigen F
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 3D11 specifically detects human HLA-F. It targets an epitope with in the alpha 2 domain from the N-terminal half.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: HLA-F
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: HLA-F;HLA-5.4;HLAF
Immunogen: Full-length human recombinant HLA-F Heavy chains (excluding the signal sequence).
Quantity: 25 μg
Research Discipline: Inflammation & Immunology
Gene ID (Entrez): NP_061823
Target Species: Human
Form: Purified
Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Clone: 3D11
Dilution: ELISA Analysis: A representative lot detected HLA-F in ELISA applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Western Blotting Analysis: A representative lot detected HLA-F in Western Blotting applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18; Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).Flow Cytometry Analysis: A representative lot detected HLA-F in Flow Cytometry applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Immunohistochemistry Analysis: A representative lot detected HLA-F in Immunohistochemistry applications (Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).
Gene Alias: HLA class I histocompatibility antigen alpha chain F;CDA12;HLA F antigen;Leukocyte antigen F;MHC class I antigen F
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 3D11 specifically detects human HLA-F. It targets an epitope with in the alpha 2 domain from the N-terminal half.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
HLA-F
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
HLA-F;HLA-5.4;HLAF
Immunogen:
Full-length human recombinant HLA-F Heavy chains (excluding the signal sequence).
Quantity:
25 μg
Research Discipline:
Inflammation & Immunology
Gene ID (Entrez):
NP_061823
Target Species:
Human
Form:
Purified
Applications:
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Clone:
3D11
Dilution:
ELISA Analysis: A representative lot detected HLA-F in ELISA applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Western Blotting Analysis: A representative lot detected HLA-F in Western Blotting applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18; Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).Flow Cytometry Analysis: A representative lot detected HLA-F in Flow Cytometry applications (Lee, N., et. al. (2010). Eur J Immunol. 40(8):2308-18).Immunohistochemistry Analysis: A representative lot detected HLA-F in Immunohistochemistry applications (Ishitani, A., et. al. (2003). J Immunol. 171(3):1376-84).
Gene Alias:
HLA class I histocompatibility antigen alpha chain F;CDA12;HLA F antigen;Leukocyte antigen F;MHC class I antigen F
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 3D11 specifically detects human HLA-F. It targets an epitope with in the alpha 2 domain from the N-terminal half.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: CD33
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene Symbols: CD33;SIGLEC3
Immunogen: FMY9S5 cells expressing CD33.
Quantity: 100 μg
Research Discipline: Inflammation & Immunology
Gene ID (Entrez): NP_001763.3
Target Species: Human
Form: Purified
Applications: Flow Cytometry, Immunocytochemistry, Inhibition Assays
Clone: P67.6
Dilution: Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58). Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
Gene Alias: Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone P67.6 specifically detects human CD 33.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG1 κ
Antigen:
CD33
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene Symbols:
CD33;SIGLEC3
Immunogen:
FMY9S5 cells expressing CD33.
Quantity:
100 μg
Research Discipline:
Inflammation & Immunology
Gene ID (Entrez):
NP_001763.3
Target Species:
Human
Form:
Purified
Applications:
Flow Cytometry, Immunocytochemistry, Inhibition Assays
Clone:
P67.6
Dilution:
Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58). Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
Gene Alias:
Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone P67.6 specifically detects human CD 33.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype:
IgG1 κ
Antigen: CD33
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene Symbols: CD33;SIGLEC3
Immunogen: FMY9S5 cells expressing CD33.
Quantity: 25 μg
Research Discipline: Inflammation & Immunology
Gene ID (Entrez): NP_001763.3
Target Species: Human
Form: Purified
Applications: Flow Cytometry, Immunocytochemistry, Inhibition Assays
Clone: P67.6
Dilution: Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58). Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
Gene Alias: Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone P67.6 specifically detects human CD 33.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG1 κ
Antigen:
CD33
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in PBS without azide.
Gene Symbols:
CD33;SIGLEC3
Immunogen:
FMY9S5 cells expressing CD33.
Quantity:
25 μg
Research Discipline:
Inflammation & Immunology
Gene ID (Entrez):
NP_001763.3
Target Species:
Human
Form:
Purified
Applications:
Flow Cytometry, Immunocytochemistry, Inhibition Assays
Clone:
P67.6
Dilution:
Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58). Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
Gene Alias:
Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone P67.6 specifically detects human CD 33.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype:
IgG1 κ