MABF208525

CD11b/CD18, Mouse anti-Human, Non-human Primate, Clone: mAb107, Millipore Sigma™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 MABF208525-Each-of-1 In Stock ₹ 16,456.61

MABF208525 - Each of 1

₹ 16,456.61

In Stock

Quantity

1

Base Price: ₹ 16,456.61

GST (18%): ₹ 2,962.19

Total Price: ₹ 19,418.80

Antigen

CD11b/CD18

Classification

Monoclonal

Conjugate

Unconjugated

Formulation

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene Symbols

ITGAM;CD11B;CR3A

Immunogen

GST-tagged recombinant fragment corresponding to 211 amino acids from the N-terminal half of human CD11bA.

Quantity

25 μg

Research Discipline

Cell Structure

Gene ID (Entrez)

NP_000623

Target Species

Human, Non-human Primate

Form

Purified

Applications

Dot Blot, Flow Cytometry, Immunohistochemistry, Inhibition Assays

Clone

mAb107

Dilution

Dot Blot Analysis: A representative lot detected CD11b/CD18 in Dot Blot applications (Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Flow Cytometry Analysis: A representative lot detected CD11b/CD18 in Flow Cytometry applications (Mahalingam, B., et. al. (2011). J Immunol. 187(12):6393-401; Li, R., et. al. (2002). J Immunol. 168(3):1219-25).Inhibition Analaysis: A representative lot prophylactically inactivated CD11b/CD18 and prevents CD11b/CD18 binding to multiple ligands (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899; Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Immunohistochemistry Analysis: A representative lot detected CD11b/CD18 in Immunohistochemistry applications (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899).

Gene Alias

Integrin alpha-M/ITGB2;CR3

Host Species

Mouse

Purification Method

Protein G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Clone mAb 107 detects CD11b/CD18 in human neutrophils. It targets an epitope with in the A domain.

Content And Storage

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Isotype

IgG1 κ

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Description

  • Anti-CD11b/CD18, clone mAb107, Cat
  • No
  • MABF2085, is a mouse monoclonal antibody that detects CD11b/CD18 nd has been tested for use in Dot Blot, Flow Cytometry, Immunohistochemistry, and Inhibition assay
  • Integrin alpha-M (UniProt: P11215; also known as CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b) is encoded by the ITGAM (also known as CD11B, CR3A) gene (Gene ID: 3684) in human
  • Integrins are alpha/beta heterodimeric adhesion receptors that couple the extracellular matrix on other cells with the contractile cytoskeleton to transduce mechanochemical signals across the plasma membrane
  • CD11b is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-16), which is cleaved off in the mature form
  • It is predominantly expressed in monocytes and granulocytes and some expression is also observed in neutrophils
  • It is usually found as a heterodimer of alpha and beta subunits and associates with Integrin beta-2 (ITGB2)
  • Two isoforms of CD11b have been reported that are produced by alternative splicing
  • CD11b contains a large extracellular domain (aa 17-1104), a short transmembrane domain (aa 1105-1128), and a cytoplasmic domain (aa 1129-1152)
  • It has been implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles
  • It contains a metal ion-dependent adhesion site (MIDAS) in the integrin A domain, which is essential for stabilization of domain A in high affinity state
  • The major ligand recognition site of CD11b/CD18 has been mapped to the A-domain in the CD11b subunit (CD11bA)
  • Both magnesium and calcium ions exhibit equal affinities for the inactive CD11bA, however, upon activation there is a 10-fold increase in the binding affinity of magnesium ions without affecting affinity of calcium ions
  • Mutations in ITGAM gene are known to cause Systemic lupus erythematosus 6, which is characterized by wide range of system dysfunctions, principally involving skin, joints, kidneys and serosal membranes
  • (Ref.: Zerria, K., et al
  • (2006)
  • Immunology 119(4); 431-440; Mahalingam, B., et al., (2011)
  • J
  • Immunol
  • 187 (12); 6393-6401; Li, R., et al
  • (2002)
  • J
  • Immunol
  • 168 (3); 1219-1225.).

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Show Difference

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MABF208525

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Antigen:
CD11b/CD18

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Gene Symbols:
ITGAM;CD11B;CR3A

Immunogen:
GST-tagged recombinant fragment corresponding to 211 amino acids from the N-terminal half of human CD11bA.

Quantity:
25 μg

Research Discipline:
Cell Structure

Gene ID (Entrez):
NP_000623

Target Species:
Human, Non-human Primate

Form:
Purified

Applications:
Dot Blot, Flow Cytometry, Immunohistochemistry, Inhibition Assays

Clone:
mAb107

Dilution:
Dot Blot Analysis: A representative lot detected CD11b/CD18 in Dot Blot applications (Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Flow Cytometry Analysis: A representative lot detected CD11b/CD18 in Flow Cytometry applications (Mahalingam, B., et. al. (2011). J Immunol. 187(12):6393-401; Li, R., et. al. (2002). J Immunol. 168(3):1219-25).Inhibition Analaysis: A representative lot prophylactically inactivated CD11b/CD18 and prevents CD11b/CD18 binding to multiple ligands (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899; Li, R., et. al. (2002). J Immunol. 168(3):1219-25). Immunohistochemistry Analysis: A representative lot detected CD11b/CD18 in Immunohistochemistry applications (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899).

Gene Alias:
Integrin alpha-M/ITGB2;CR3

Host Species:
Mouse

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Clone mAb 107 detects CD11b/CD18 in human neutrophils. It targets an epitope with in the A domain.

Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Isotype:
IgG1 κ

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Antigen:
Adenylate Cyclase Toxin

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
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Gene Symbols:
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Immunogen:
Adenylate cyclase (AC) toxin from Bordetella pertussis.

Quantity:
100 μg

Research Discipline:
Inflammation and Immunology

Gene ID (Entrez):
__

Target Species:
Bacteria

Form:
Purified

Applications:
ELISA, Flow Cytometry, Inhibition Assays, Neutralization, Western Blot

Clone:
3D1

Dilution:
__

Gene Alias:
__

Host Species:
Mouse

Purification Method:
Protein G Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Clone 3D1 is a mouse monoclonal antibody that detects Adenylate cyclase (AC) toxin from Bordetella pertussis.

Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Isotype:
IgG1 κ

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MABF2086-25

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Antigen:
Adenylate Cyclase Toxin

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
__

Gene Symbols:
__

Immunogen:
Adenylate cyclase (AC) toxin from Bordetella pertussis.

Quantity:
25 μg

Research Discipline:
Inflammation and Immunology

Gene ID (Entrez):
__

Target Species:
Bacteria

Form:
Purified

Applications:
ELISA, Flow Cytometry, Inhibition Assays, Neutralization, Western Blot

Clone:
3D1

Dilution:
__

Gene Alias:
__

Host Species:
Mouse

Purification Method:
Protein G Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Clone 3D1 is a mouse monoclonal antibody that detects Adenylate cyclase (AC) toxin from Bordetella pertussis.

Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Isotype:
IgG1 κ

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Antigen:
Adenylate Cyclase Toxin

Classification:
Monoclonal

Conjugate:
Unconjugated

Formulation:
__

Gene Symbols:
__

Immunogen:
Adenylate cyclase toxin from Bordetella pertussis.

Quantity:
100 μL

Research Discipline:
Inflammation and Immunology

Gene ID (Entrez):
__

Target Species:
Bacteria

Form:
Purified

Applications:
Dot Blot, Functional Assay, Western Blot

Clone:
9D4

Dilution:
__

Gene Alias:
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Host Species:
Mouse

Purification Method:
Protein G Purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Clone 9D4 is a mouse monoclonal antibody that detects Adenylate cyclase (AC) toxin from Bordetella pertussis.

Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Isotype:
IgG2a λ