MABS199025
RhoB, Mouse anti-Monkey, Porcine, Rat, Chicken, Human, Mouse, Rabbit, Hamster, Clone: 7F7, Millipore Sigma™
Manufacturer: MilliporeSigma
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABS199025-Each-of-1 | In Stock | ₹ 16,456.61 |
MABS199025 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
RhoB
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
RHOB;ARH6;ARHB
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human RhoB.
Quantity
25 μL
Research Discipline
Signaling
Test Specificity
Clone 7F7 detects RhoB in multiple species. It targets an epitope within 19 amino acids from the C-terminal half. This clone specifically detects RhoB, but not RhoA, Rac1, or cdc42.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1
Applications
ELISA, Western Blot
Clone
7F7
Dilution
Western Blotting Analysis: A 1:2,000 dilution from a representative lotdetected RhoB in 10μg of MCF7 cell lysate. Western Blotting Analysis: A representative lot detected RhoB in mouse and rabbit spleen, mouse kidney, recombinant RhoB, 293 cells expressing RhoB, CHO, H9C2, PK1P.185, COS, MDA MB-231, MCF7 and chicken fibroblasts (Courtesy of Lisa Laury-Kleintop, Ph.D. , Lankenau Institute for Medical Research, Philadelphia PA, USA). ELISA Analysis: A representative lot detected RhoB in Plate bound recombinant RhoB (Courtesy of Lisa Laury-Kleintop, Ph.D. , Lankenau Institute for Medical Research, Philadelphia PA, USA).
Gene Alias
Rho-related GTP-binding protein RhoB;Rho cDNA clone 6;h6
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Monkey, Porcine, Rat, Chicken, Human, Mouse, Rabbit, Hamster
Form
Purified
Description
- Anti-RhoB, clone 7F7, Cat
- No
- MABS1990, is a mouse monoclonal antibody that detects Rho-related GTP-binding protein RhoB in human and has been tested for use in ELISA and Western Blotting
- Rho-related GTP-binding protein RhoB (UniProt: P62745; also known as Rho cDNA clone 6, h6) is encoded by the RHOB (also known as ARH6, ARHB) gene (Gene ID: 388) in human
- RhoB is a member of the Ras super-family of small GTP-binding proteins, which is involved in hydrolysis of GTP and is active in GTP-bound form
- RhoB is a short-lived Rho GTPase whose expression is inducible by a variety of stimuli including growth factors and stress stimuli
- Its levels are up-regulated by DNA damaging agents, such as hydrogen peroxide or ionizing radiation
- RhoB plays a negative role in tumorigenesis as its deletion is known to cause tumor formation
- It is reported to mediate apoptosis in neoplastically transformed cells following DNA damage
- It is not considered to be essential for development, but affects cell adhesion and growth factor signaling in transformed cells
- RhoB displays 83% homology with RhoA
- However, unlike RhoA, which is cytosolic and translocates to plasma membrane upon activation, RhoB localizes to endosomes/multivesicular bodies
- RhoB participates in sorting and degradation of growth factors and cytokine receptors
- RhoB has three nucleotide-binding regions (aa 12-19; 59-63; and 117-120)
- RhoB is shown to be critically required for the inflammatory response of endothelial cells to TNF alpha, possibly through MAP kinase activation downstream of the TNFR.
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Antigen: RhoB
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: RHOB;ARH6;ARHB
Immunogen: KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human RhoB.
Quantity: 25 μL
Research Discipline: Signaling
Test Specificity: Clone 7F7 detects RhoB in multiple species. It targets an epitope within 19 amino acids from the C-terminal half. This clone specifically detects RhoB, but not RhoA, Rac1, or cdc42.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1
Applications: ELISA, Western Blot
Clone: 7F7
Dilution: Western Blotting Analysis: A 1:2,000 dilution from a representative lotdetected RhoB in 10μg of MCF7 cell lysate. Western Blotting Analysis: A representative lot detected RhoB in mouse and rabbit spleen, mouse kidney, recombinant RhoB, 293 cells expressing RhoB, CHO, H9C2, PK1P.185, COS, MDA MB-231, MCF7 and chicken fibroblasts (Courtesy of Lisa Laury-Kleintop, Ph.D. , Lankenau Institute for Medical Research, Philadelphia PA, USA). ELISA Analysis: A representative lot detected RhoB in Plate bound recombinant RhoB (Courtesy of Lisa Laury-Kleintop, Ph.D. , Lankenau Institute for Medical Research, Philadelphia PA, USA).
Gene Alias: Rho-related GTP-binding protein RhoB;Rho cDNA clone 6;h6
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Monkey, Porcine, Rat, Chicken, Human, Mouse, Rabbit, Hamster
Form: Purified
Antigen:
RhoB
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
RHOB;ARH6;ARHB
Immunogen:
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human RhoB.
Quantity:
25 μL
Research Discipline:
Signaling
Test Specificity:
Clone 7F7 detects RhoB in multiple species. It targets an epitope within 19 amino acids from the C-terminal half. This clone specifically detects RhoB, but not RhoA, Rac1, or cdc42.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1
Applications:
ELISA, Western Blot
Clone:
7F7
Dilution:
Western Blotting Analysis: A 1:2,000 dilution from a representative lotdetected RhoB in 10μg of MCF7 cell lysate. Western Blotting Analysis: A representative lot detected RhoB in mouse and rabbit spleen, mouse kidney, recombinant RhoB, 293 cells expressing RhoB, CHO, H9C2, PK1P.185, COS, MDA MB-231, MCF7 and chicken fibroblasts (Courtesy of Lisa Laury-Kleintop, Ph.D. , Lankenau Institute for Medical Research, Philadelphia PA, USA). ELISA Analysis: A representative lot detected RhoB in Plate bound recombinant RhoB (Courtesy of Lisa Laury-Kleintop, Ph.D. , Lankenau Institute for Medical Research, Philadelphia PA, USA).
Gene Alias:
Rho-related GTP-binding protein RhoB;Rho cDNA clone 6;h6
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Monkey, Porcine, Rat, Chicken, Human, Mouse, Rabbit, Hamster
Form:
Purified
Antigen: MT-ND4
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.
Gene Symbols: MT-ND4;MTND4;NADH4;ND4
Immunogen: KLH-conjugated linear peptide from the C-terminal region.
Quantity: 25 μg
Research Discipline: Signaling
Test Specificity: Clone 9E4-2D8 detects NADH-ubiquinone oxidoreductase chain 4 in mitochodria isolated from human cells.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2a
Applications: Western Blot
Clone: 9E4-2D8
Dilution: Western Blotting Analysis: 4μg/mL from a representative lot detected MT-ND4 in mitochondria from Human Neonatal Dermal Fibroblasts and mitochondria from Human neonatal dermal fibroblasts depleted of mtDNA. (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR, USA ).
Gene Alias: NADH-ubiquinone oxidoreductase chain 4;EC: 1.6.5.3;NADH dehydrogenase subunit 4
Host Species: Mouse
Purification Method: Protein L
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
MT-ND4
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.
Gene Symbols:
MT-ND4;MTND4;NADH4;ND4
Immunogen:
KLH-conjugated linear peptide from the C-terminal region.
Quantity:
25 μg
Research Discipline:
Signaling
Test Specificity:
Clone 9E4-2D8 detects NADH-ubiquinone oxidoreductase chain 4 in mitochodria isolated from human cells.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2a
Applications:
Western Blot
Clone:
9E4-2D8
Dilution:
Western Blotting Analysis: 4μg/mL from a representative lot detected MT-ND4 in mitochondria from Human Neonatal Dermal Fibroblasts and mitochondria from Human neonatal dermal fibroblasts depleted of mtDNA. (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR, USA ).
Gene Alias:
NADH-ubiquinone oxidoreductase chain 4;EC: 1.6.5.3;NADH dehydrogenase subunit 4
Host Species:
Mouse
Purification Method:
Protein L
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Antigen: MT-ATP6
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2b in HEPES-Buffered Saline (15 mM HEPES, 150 mM NaCl, pH 7.2) with 0.02% sodium azide.
Gene Symbols: MT-ATP6;ATP6;ATPASE6;MTATP6
Immunogen: A synthetic peptide corresponding to the N-terminus of human MT-ATP6.
Quantity: 25 μg
Research Discipline: Signaling
Test Specificity: Clone 1G7-1G2 detects ATP synthase subunit a (MT-ATP6) in mitochondria isolated from human cells.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2b κ
Applications: Western Blot
Clone: 1G7-1G2
Dilution: Western Blotting Analysis: 0.5μg/mL from a representative lot detected MT-ATP6 in mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR USA).
Gene Alias: ATP synthase subunit a;F-ATPase protein 6
Host Species: Mouse
Purification Method: Protein L
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
MT-ATP6
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG2b in HEPES-Buffered Saline (15 mM HEPES, 150 mM NaCl, pH 7.2) with 0.02% sodium azide.
Gene Symbols:
MT-ATP6;ATP6;ATPASE6;MTATP6
Immunogen:
A synthetic peptide corresponding to the N-terminus of human MT-ATP6.
Quantity:
25 μg
Research Discipline:
Signaling
Test Specificity:
Clone 1G7-1G2 detects ATP synthase subunit a (MT-ATP6) in mitochondria isolated from human cells.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2b κ
Applications:
Western Blot
Clone:
1G7-1G2
Dilution:
Western Blotting Analysis: 0.5μg/mL from a representative lot detected MT-ATP6 in mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR USA).
Gene Alias:
ATP synthase subunit a;F-ATPase protein 6
Host Species:
Mouse
Purification Method:
Protein L
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Antigen: ST8SIA1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Gene Symbols: ST8SIA1;SIAT8;SIAT8A
Immunogen: Trypsin-dissociated human pancreatic islet cells with low levels of exocrine and ductal cells.
Quantity: 100 μg
Research Discipline: Signaling
Test Specificity: Clone HIC0-3C5 specifically detects human Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (ST8SIA1).
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgM κ
Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry
Clone: HIC0-3C5
Dilution: Flow Cytometry Analysis: A representative lot detected ST8SIA1 in Flow Cytometry applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).Immunofluorescence Analysis: A representative lot detected ST8SIA1 in Immunofluorescence applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
Gene Alias: Alpha-N-acetylneuraminide alpha-2;8-sialyltransferase;EC: 2.4.99.8;Alpha-2;8-sialyltransferase 8A;Ganglioside GD3 synthase;Ganglioside GT3 synthase;Sialyltransferase 8A;SIAT8-A;Sialyltransferase St8Sia I;ST8SiaI
Host Species: Mouse
Purification Method: Purified by ion-exchange chromatography
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
ST8SIA1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
Gene Symbols:
ST8SIA1;SIAT8;SIAT8A
Immunogen:
Trypsin-dissociated human pancreatic islet cells with low levels of exocrine and ductal cells.
Quantity:
100 μg
Research Discipline:
Signaling
Test Specificity:
Clone HIC0-3C5 specifically detects human Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (ST8SIA1).
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgM κ
Applications:
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Clone:
HIC0-3C5
Dilution:
Flow Cytometry Analysis: A representative lot detected ST8SIA1 in Flow Cytometry applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).Immunofluorescence Analysis: A representative lot detected ST8SIA1 in Immunofluorescence applications (Dorrell, C., et. al. (2016). Nat Commun. 7:11756).
Gene Alias:
Alpha-N-acetylneuraminide alpha-2;8-sialyltransferase;EC: 2.4.99.8;Alpha-2;8-sialyltransferase 8A;Ganglioside GD3 synthase;Ganglioside GT3 synthase;Sialyltransferase 8A;SIAT8-A;Sialyltransferase St8Sia I;ST8SiaI
Host Species:
Mouse
Purification Method:
Purified by ion-exchange chromatography
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified