MABT85125UG
Podoplanin, Rat anti-Mouse, Clone: PMab-1, Millipore Sigma™
Manufacturer: MilliporeSigma
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT85125UG-Each-of-1 | In Stock | ₹ 16,456.61 |
MABT85125UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
Podoplanin
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
Pdpn;Gp38;Ots8
Immunogen
KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of murine Podoplanin.
Quantity
25 μg
Research Discipline
Cell Structure
Test Specificity
Clone PMab-1 detects murine Podoplanin. It targets an epitipe within 14 amino acids from the N-terminal region.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG2a κ
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone
PMab-1
Dilution
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in mouse lung tissue. Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kaji, C., et. al. (2012) Acta Histochem Cytochem . 45(4):227-37). Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother . 34(6):396-403). Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother . 34(6):396-403). Immunocytochemistry Analysis: A representative lot detected Podoplanin in Immunocytochemistry applications (Kaji, C., et. al. (2012) Acta Histochem Cytochem . 45(4):227-37).
Gene Alias
Aggrus;Glycoprotein 38;Gp38;OTS-8;PA2.26 antigen;T1-alpha;T1A;Transmembrane glycoprotein E11
Host Species
Rat
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Mouse
Form
Purified
Description
- Anti-Podoplanin, clone PMab-1, Cat
- No
- MABT851, is a rat monoclonal antibody that detects mouse Podoplanin and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting
- Podoplanin (UniProt: Q62011; also known as Aggrus, Glycoprotein 38, Gp38, OTS-8, PA2.26 antigen, T1-alpha, T1A, Transmembrane glycoprotein E11) I encoded by the Pdpn (also known as Gp38, Ots8) gene (Gene ID: 14726) in murine species
- Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes
- Podoplanin is detected at high levels in lung and brain, at lower levels in kidney, stomach, liver, spleen and esophagus, and not detected in skin and small intestine
- It is expressed in epithelial cells of choroid plexus, ependyma, glomerulus and alveolus, in mesothelial cells and in endothelia of lymphatic vessels
- It is also expressed in stromal cells of peripheral lymphoid tissue and thymic epithelial cells and is detected in carcinoma cell lines and cultured fibroblasts
- High expression of Podoplanin is observed in colon carcinomas
- It may be involved in cell migration and/or actin cytoskeleton organization
- Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles
- Extensively O-glycosylated
- Contains sialic acid residues
- O-glycosylation is necessary for platelet aggregation activity
- Podoplanin levels are down-regulated by treatment with puromycin aminonucleoside
- Mice lacking or with defective podoplanin die at birth of respiratory failure due to a low number of attenuated type I cells, narrow and irregular air spaces, and defective formation of alveolar saccules
- Administration of PMab-1 antibody is shown to reduce lymphangiogenesis in the corneal suture and ear wound healing murine models
- It suppresses thioglycollate-induced macrophages infiltration at the site of wound healing
- (Ref.: Maruyama, Y et al (2014)
- Invest.Ophthal
- Visual Sci
- 55(8); 4813-4822).
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Antigen: Podoplanin
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: Pdpn;Gp38;Ots8
Immunogen: KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of murine Podoplanin.
Quantity: 25 μg
Research Discipline: Cell Structure
Test Specificity: Clone PMab-1 detects murine Podoplanin. It targets an epitipe within 14 amino acids from the N-terminal region.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2a κ
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone: PMab-1
Dilution: Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in mouse lung tissue. Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kaji, C., et. al. (2012) Acta Histochem Cytochem . 45(4):227-37). Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother . 34(6):396-403). Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother . 34(6):396-403). Immunocytochemistry Analysis: A representative lot detected Podoplanin in Immunocytochemistry applications (Kaji, C., et. al. (2012) Acta Histochem Cytochem . 45(4):227-37).
Gene Alias: Aggrus;Glycoprotein 38;Gp38;OTS-8;PA2.26 antigen;T1-alpha;T1A;Transmembrane glycoprotein E11
Host Species: Rat
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Mouse
Form: Purified
Antigen:
Podoplanin
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
Pdpn;Gp38;Ots8
Immunogen:
KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of murine Podoplanin.
Quantity:
25 μg
Research Discipline:
Cell Structure
Test Specificity:
Clone PMab-1 detects murine Podoplanin. It targets an epitipe within 14 amino acids from the N-terminal region.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2a κ
Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone:
PMab-1
Dilution:
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in mouse lung tissue. Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kaji, C., et. al. (2012) Acta Histochem Cytochem . 45(4):227-37). Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother . 34(6):396-403). Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother . 34(6):396-403). Immunocytochemistry Analysis: A representative lot detected Podoplanin in Immunocytochemistry applications (Kaji, C., et. al. (2012) Acta Histochem Cytochem . 45(4):227-37).
Gene Alias:
Aggrus;Glycoprotein 38;Gp38;OTS-8;PA2.26 antigen;T1-alpha;T1A;Transmembrane glycoprotein E11
Host Species:
Rat
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Mouse
Form:
Purified
Antigen: __
Classification: __
Conjugate: __
Formulation: __
Gene Symbols: __
Immunogen: __
Quantity: __
Research Discipline: __
Test Specificity: __
Content And Storage: __
Isotype: __
Applications: __
Clone: __
Dilution: __
Gene Alias: __
Host Species: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Target Species: __
Form: __
Antigen:
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Classification:
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Conjugate:
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Formulation:
__
Gene Symbols:
__
Immunogen:
__
Quantity:
__
Research Discipline:
__
Test Specificity:
__
Content And Storage:
__
Isotype:
__
Applications:
__
Clone:
__
Dilution:
__
Gene Alias:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Target Species:
__
Form:
__
Antigen: Podoplanin
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: PDPN;GP36
Immunogen: LN229 glioma cells transfected with human podoplanin.
Quantity: 25 μg
Research Discipline: Cell Structure
Test Specificity: Clone LpMab-21 detects human podoplanin. It targets a minimum epitope Thr76-Arg79 witih sialic acid linked to Thr76.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2a κ
Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin)
Clone: LpMab-21
Dilution: Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kaneko, M.K., et. al. (2017). Cancer Med. 6(2):382-396).ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kaneko, M.K., et. al. (2017). Cancer Med. 6(2):382-396).Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kaneko, M.K., et. al. (2017). Cancer Med. 6(2):382-396).
Gene Alias: Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
Podoplanin
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
PDPN;GP36
Immunogen:
LN229 glioma cells transfected with human podoplanin.
Quantity:
25 μg
Research Discipline:
Cell Structure
Test Specificity:
Clone LpMab-21 detects human podoplanin. It targets a minimum epitope Thr76-Arg79 witih sialic acid linked to Thr76.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2a κ
Applications:
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin)
Clone:
LpMab-21
Dilution:
Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kaneko, M.K., et. al. (2017). Cancer Med. 6(2):382-396).ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kaneko, M.K., et. al. (2017). Cancer Med. 6(2):382-396).Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kaneko, M.K., et. al. (2017). Cancer Med. 6(2):382-396).
Gene Alias:
Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Antigen: Podoplanin
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: PDPN;GP36
Immunogen: LN229 glioma cells expressiing human Podoplanin.
Quantity: 25 μg
Research Discipline: Cell Structure
Test Specificity: Clone LpMab-19 detects Podoplanin in human cells. It recognizes sialylated O-Glycan on Thr76. The minimal epitope of LpMab-19 is identified as Thr76-Arg79.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2b κ
Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin)
Clone: LpMab-19
Dilution: Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in human colon cancer tissue. ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253. Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253. Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253.
Gene Alias: Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
Podoplanin
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
PDPN;GP36
Immunogen:
LN229 glioma cells expressiing human Podoplanin.
Quantity:
25 μg
Research Discipline:
Cell Structure
Test Specificity:
Clone LpMab-19 detects Podoplanin in human cells. It recognizes sialylated O-Glycan on Thr76. The minimal epitope of LpMab-19 is identified as Thr76-Arg79.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2b κ
Applications:
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin)
Clone:
LpMab-19
Dilution:
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in human colon cancer tissue. ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253. Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253. Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(5); 245-253.
Gene Alias:
Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified