prelamin A, Mouse anti-Mouse, Human, Clone: PL-1C7, Millipore Sigma™
Manufacturer: MilliporeSigma
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABT85825UG-Each-of-1 | In Stock | ₹ 16,456.61 |
MABT85825UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
prelamin A
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
LMNA;LMN1
Immunogen
A lnear peptide corresponding to 12 amino acids from the C-terminal region of human Prelamin A.
Quantity
25 μg
Research Discipline
Cell Structure
Test Specificity
Clone PL-1C7 detects Prelamin A in human and murine cells. It specifically recognizes prelamin A at the intact ZMPSTE24 cleavage site.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG2b
Applications
ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Clone
PL-1C7
Dilution
Western Blotting Analysis: 2 μg/mL from a representative lot detected prelamin A in U20S cells treated with farnesyl transferase inhibitor, Lonafarnib (3.2 uM for 24 h) . Flow Cytometry Analysis: A representative lot detected prelamin A in Flow Cytometry applications (Casasola, A., et. al. (2016). Nucleus. 7(1):84-102). Western Blotting Analysis: A representative lot detected prelamin A in Western Blotting applications (Casasola, A., et. al. (2016). Nucleus. 7(1):84-102). Immunocytochemistry Analysis: 1μg/mL from a representative lot detected prelamin A in C2C12 cells with Farnesyl transferase inhibitor Lonafarnib (Courtesy of Fred Hutchinson Cancer Research Center, Seattle, Washington USA). ELISA Analysis: A representative lot detected prelamin A in ELISA applications (Casasola, A., et. al. (2016). Nucleus. 7(1):84-102). Immunocytochemistry Analysis: A representative lot detected prelamin A in Immunocytochemistry applications (Casasola, A., et. al. (2016). Nucleus.
Gene Alias
LMNA
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Mouse, Human
Form
Purified
Description
- Anti-prelamin A, clone PL-1C7, Cat
- No
- MABT858, is a mouse monoclonal antibody that detects Prelamin A and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting
- Prelamin-A/C (UniProt: P02545; also known as 70 kDa lamin, Renal carcinoma antigen NY-REN-32) is encoded by the LMNA (also known as LMN1) gene (Gene ID: 4000) in human
- Lamins are components of the nuclear lamina that provide a framework for the nuclear envelope and may also interact with chromatin
- Lamin A and C are present in equal amounts in the lamina of mammals
- Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics
- Lamin A is initially synthesized as prelamin A that undergoes several modifications in the carboxyl terminal region that allow incorporation of prelamin A into the nuclear envelope and its subsequent processing into the mature lamin A
- Cleavage of 15 residues (aa 647-662) by ZMPSTE24/FACE1 generates the final protein product
- Unlike mature lamin A, prelamin A accumulates as discrete and localized foci at the nuclear periphery
- Prelamin-A/C can accelerate smooth muscle cell senescence
- It can act to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence
- Mutations in LMNA gene are known to cause Emery-Dreifuss muscular dystrophy that is characterized by weakness and atrophy of muscle without involvement of the nervous system
- Some mutations have also been linked to familial type of lipodystrophy characterized by the loss of subcutaneous adipose tissue in the lower parts of the body
- (Ref.: Casasola, A., et al
- (2016)
- Nucleus 7(1); 84-102).
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Antigen: prelamin A
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: LMNA;LMN1
Immunogen: A lnear peptide corresponding to 12 amino acids from the C-terminal region of human Prelamin A.
Quantity: 25 μg
Research Discipline: Cell Structure
Test Specificity: Clone PL-1C7 detects Prelamin A in human and murine cells. It specifically recognizes prelamin A at the intact ZMPSTE24 cleavage site.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2b
Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Clone: PL-1C7
Dilution: Western Blotting Analysis: 2 μg/mL from a representative lot detected prelamin A in U20S cells treated with farnesyl transferase inhibitor, Lonafarnib (3.2 uM for 24 h) . Flow Cytometry Analysis: A representative lot detected prelamin A in Flow Cytometry applications (Casasola, A., et. al. (2016). Nucleus. 7(1):84-102). Western Blotting Analysis: A representative lot detected prelamin A in Western Blotting applications (Casasola, A., et. al. (2016). Nucleus. 7(1):84-102). Immunocytochemistry Analysis: 1μg/mL from a representative lot detected prelamin A in C2C12 cells with Farnesyl transferase inhibitor Lonafarnib (Courtesy of Fred Hutchinson Cancer Research Center, Seattle, Washington USA). ELISA Analysis: A representative lot detected prelamin A in ELISA applications (Casasola, A., et. al. (2016). Nucleus. 7(1):84-102). Immunocytochemistry Analysis: A representative lot detected prelamin A in Immunocytochemistry applications (Casasola, A., et. al. (2016). Nucleus.
Gene Alias: LMNA
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Mouse, Human
Form: Purified
Antigen: CDCP1 (CD318)
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: CDCP1;TRASK
Immunogen: His-tagged recombinant fragment corresponding to 638 amino acids from the extracellular domain of human CUB domain-containing protein 1.
Quantity: 25 μg
Research Discipline: Cell Structure
Test Specificity: Clone 8A2 detects CUB domain-containing protein 1 (CDCP1) in human cells. it targets an epitope within the extracellular domain.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2b κ
Applications: Flow Cytometry
Clone: 8A2
Dilution: Flow Cytometry Analysis: A representative lot detected CDCP1 (CD318) in one million MDA and MDA KO cells (C ourtesy of the Laboratory of Feng Lin, Ph.D., Cleveland Clinic, Lerner Research Institute, Cleveland OH USA ).
Gene Alias: CUB domain-containing protein 1;Membrane glycoprotein gp140;Subtractive immunization M plus HEp3-associated 135 kDa protein;SIMA135;Transmembrane and associated with src kinases;CD318
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
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