Epredia™ Lab Vision™ Retinoblastoma (Rb) Ab-1 Mouse Monoclonal Antibody, 200μg/mL, biotinylated, BSA and azide

Description

• Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA
• It is phosphorylated on serine and threonine, but not on tyrosine residues
• It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E
• Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth
• Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia
• Host Species: Mouse Clone: 1F8; same as Rb1 Isotype: IgG1 Species Reactivity: Human
• Does not react with mouse and rat
• Others not known
• Epitope: aa 703-722 Immunogen: Recombinant human Rb protein Molecular Weight: 105kDa Positive Control: Ls174T, HT29, HUVEC cells; Normal colon, Breast CA and colon CA Cellular Localization: Nuclear Ab-1 specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells
• It reacts with hyperphosphorylated and un (under) phosphorylated form of Rb protein
• It shows no cross reaction with p107 and p130
• Recommended for: Immunohistochemistry (Formalin/paraffin) Western Blotting Immunofluorescence Immunoprecipitation (Native and denatured) Flow Cytometry Note: USA: RUO; Int'l: RUO