Pds1 Ab-1 Mouse Monoclonal Antibody, Epredia™

Description

• Pds1 is an anaphase inhibitor and plays important role in DNA damage and spindle check point pathways
• Pds1 inhibits sister chromatid separation by binding and inhibiting Esp1, cystein protease that causes cleavage of the cohesin Scc1 that binds the sister chromatids together
• Pds1 is responsible for targeting of Esp1 to nucleus and its binding to the spindle
• Degradation of Pds1 occurs shortly before anaphase, which liberates Esp1 and is a prerequisite for anaphase entry
• Pds1 is targeted for degradation by ubiquitination mediated by cyclosome/anaphase-promoting complex (APC) functioning as a ubiquitin ligase
• In response to DNA damage Chk1 phosphorylates Pds1 to stabilize it against the APC mediated destruction, hence preventing the entry of such a cell into anaphase
• Host Species: Mouse Clone: DCS-280 Isotype: IgG2a/κ Species Reactivity: Human
• Others not known
• Epitope: Not determined Immunogen: Human recombinant full-length Pds1 protein Molecular Weight: 42kDa Positive Control: HeLa cells, Tonsil Cellular Localization: Nuclear Recommended for: Immunohistochemistry (Formalin/paraffin) Western Blotting (Not verified) Note: USA: RUO; Int'l: RUO