Classification:
Monoclonal
Purification Method:
Protein A or G purified
Primary or Secondary:
Primary
Target Species:
Human, Mouse
Applications:
Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Peptide Array
Dilution:
Western Blot 1-2 μg/mL, Immunohistochemistry 1-2 μg/mL, Immunohistochemistry-Paraffin 1-2 μg/mL, Protein Array
Gene Alias:
CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 molecule, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, melanoma 1 antigen, Melanoma-associated antigen ME491, melanoma-associated antigen MLA1, MLA1lysosome-associated membrane glycoprotein 3, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, tspan-30, TSPAN30granulophysin
Immunogen:
Recombinant human CD63 protein fragment (around aa100-197) (exact sequence is proprietary) (Uniprot: P08962 )
Test Specificity:
This monoclonal antibody recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of monoclonal antibody LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak
Content And Storage:
Store at 4°C.