NB008601
IgG F(ab)2 Goat anti-Mouse, DyLight 488, Polyclonal, Seconday Antibody, Novus Biologicals™
Manufacturer: Novus Biologicals
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NB008601-Each-of-1 | In Stock | ₹ 42,737.22 |
NB008601 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 42,737.22
GST (18%): ₹ 7,692.70
Total Price: ₹ 50,429.92
Antigen
IgG F(ab)2
Classification
Polyclonal
Conjugate
DyLight 488
Formulation
Phosphate Buffered Saline (PBS) containing 0.2% BSA.
Immunogen
Mouse IgG-F(ab')2 Fragment
Quantity
0.5 mL
Primary or Secondary
Secondary
Target Species
Mouse
Isotype
IgG
Applications
Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Concentration
0.5 mg/mL
Dilution
Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunofluorescence 1:50 - 1:500, Immunocytochemistry 1:50 - 1:500
Host Species
Goat
Purification Method
Affinity Purified
Regulatory Status
RUO
Test Specificity
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 488. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.
Content And Storage
Store at 4°C. Do not freeze.
Related Products
Description
- IgG F(ab')2 Polyclonal specifically detects IgG F(ab')2 in Mouse samples
- It is validated for Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry.
Compare Similar Items
Show Difference
Antigen: IgG F(ab)2
Classification: Polyclonal
Conjugate: DyLight 488
Formulation: Phosphate Buffered Saline (PBS) containing 0.2% BSA.
Immunogen: Mouse IgG-F(ab')2 Fragment
Quantity: 0.5 mL
Primary or Secondary: Secondary
Target Species: Mouse
Isotype: IgG
Applications: Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Concentration: 0.5 mg/mL
Dilution: Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunofluorescence 1:50 - 1:500, Immunocytochemistry 1:50 - 1:500
Host Species: Goat
Purification Method: Affinity Purified
Regulatory Status: RUO
Test Specificity: Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 488. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.
Content And Storage: Store at 4°C. Do not freeze.
Antigen:
IgG F(ab)2
Classification:
Polyclonal
Conjugate:
DyLight 488
Formulation:
Phosphate Buffered Saline (PBS) containing 0.2% BSA.
Immunogen:
Mouse IgG-F(ab')2 Fragment
Quantity:
0.5 mL
Primary or Secondary:
Secondary
Target Species:
Mouse
Isotype:
IgG
Applications:
Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Concentration:
0.5 mg/mL
Dilution:
Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunofluorescence 1:50 - 1:500, Immunocytochemistry 1:50 - 1:500
Host Species:
Goat
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Test Specificity:
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 488. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.
Content And Storage:
Store at 4°C. Do not freeze.
Antigen: IgG F(ab)2
Classification: Polyclonal
Conjugate: HRP
Formulation: Phosphate Buffered Saline (PBS) containing 0.2% BSA.
Immunogen: Mouse IgG-F(ab')2 Fragment
Quantity: 0.5 mL
Primary or Secondary: Secondary
Target Species: Mouse
Isotype: IgG
Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry
Concentration: 0.5 mg/mL
Dilution: Western Blot, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry 1:10-1:500
Host Species: Goat
Purification Method: Affinity Purified
Regulatory Status: RUO
Test Specificity: Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.
Content And Storage: Store at 4°C. Do not freeze.
Antigen:
IgG F(ab)2
Classification:
Polyclonal
Conjugate:
HRP
Formulation:
Phosphate Buffered Saline (PBS) containing 0.2% BSA.
Immunogen:
Mouse IgG-F(ab')2 Fragment
Quantity:
0.5 mL
Primary or Secondary:
Secondary
Target Species:
Mouse
Isotype:
IgG
Applications:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry
Concentration:
0.5 mg/mL
Dilution:
Western Blot, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry 1:10-1:500
Host Species:
Goat
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Test Specificity:
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.
Content And Storage:
Store at 4°C. Do not freeze.
Antigen: IgG F(ab)2
Classification: Polyclonal
Conjugate: R-PE
Formulation: Phosphate Buffered Saline (PBS) containing 0.2% BSA.
Immunogen: Mouse IgG-F(ab')2 Fragment
Quantity: 0.5 mL
Primary or Secondary: Secondary
Target Species: Mouse
Isotype: IgG
Applications: Flow Cytometry, Immunofluorescence
Concentration: 0.5 mg/mL
Dilution: Flow Cytometry 1:50 - 1:200, Immunofluorescence 1:50 - 1:200
Host Species: Goat
Purification Method: Affinity Purified
Regulatory Status: RUO
Test Specificity: Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to R-phycoerythrin (RPE). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.
Content And Storage: Store at 4°C. Do not freeze.
Antigen:
IgG F(ab)2
Classification:
Polyclonal
Conjugate:
R-PE
Formulation:
Phosphate Buffered Saline (PBS) containing 0.2% BSA.
Immunogen:
Mouse IgG-F(ab')2 Fragment
Quantity:
0.5 mL
Primary or Secondary:
Secondary
Target Species:
Mouse
Isotype:
IgG
Applications:
Flow Cytometry, Immunofluorescence
Concentration:
0.5 mg/mL
Dilution:
Flow Cytometry 1:50 - 1:200, Immunofluorescence 1:50 - 1:200
Host Species:
Goat
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Test Specificity:
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to R-phycoerythrin (RPE). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.
Content And Storage:
Store at 4°C. Do not freeze.
Antigen: Bromodeoxyuridine/BrdU
Classification: Monoclonal
Conjugate: Alexa Fluor 405
Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
Immunogen: Bromodeoxyuridine (BrdU) conjugated to hemocyanine (isolated from Helix pomatia)
Quantity: 0.1 mL
Primary or Secondary: Primary
Target Species: All species
Isotype: IgG1 κ
Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Concentration: __
Dilution: Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Test Specificity: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Content And Storage: Store at 4°C in the dark.
Antigen:
Bromodeoxyuridine/BrdU
Classification:
Monoclonal
Conjugate:
Alexa Fluor 405
Formulation:
50mM Sodium Borate with 0.05% Sodium Azide
Immunogen:
Bromodeoxyuridine (BrdU) conjugated to hemocyanine (isolated from Helix pomatia)
Quantity:
0.1 mL
Primary or Secondary:
Primary
Target Species:
All species
Isotype:
IgG1 κ
Applications:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Concentration:
__
Dilution:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Test Specificity:
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Content And Storage:
Store at 4°C in the dark.