NB008596

IgG F(ab)2 Goat anti-Mouse, Allophycocyanin, Polyclonal, Seconday Antibody, Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NB008596-Each-of-1 In Stock ₹ 42,737.22

NB008596 - Each of 1

₹ 42,737.22

In Stock

Quantity

1

Base Price: ₹ 42,737.22

GST (18%): ₹ 7,692.70

Total Price: ₹ 50,429.92

Antigen

IgG F(ab)2

Classification

Polyclonal

Conjugate

APC

Formulation

50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA.

Immunogen

Mouse IgG-F(ab')2 Fragment

Quantity

0.5 mL

Primary or Secondary

Secondary

Target Species

Mouse

Isotype

IgG

Applications

Western Blot, ELISA, Immunohistochemistry, Immunofluorescence

Concentration

0.5 mg/mL

Dilution

Western Blot 1:2500-1:25000, ELISA 1:2500-1:25000, Immunohistochemistry 1:100-1:1000, Immunocytochemistry/Immunofluorescence 1:100-1:1000

Host Species

Goat

Purification Method

Affinity Purified

Regulatory Status

RUO

Test Specificity

Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to alkaline phosphatase. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.

Content And Storage

Store at 4°C in the dark.

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Description

  • IgG F(ab')2 Polyclonal specifically detects IgG F(ab')2 in Mouse samples
  • It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence.

Compare Similar Items

Show Difference

Img

Novus Biologicals

NB008596

--


Antigen:
IgG F(ab)2

Classification:
Polyclonal

Conjugate:
APC

Formulation:
50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2 containing 0.2% BSA.

Immunogen:
Mouse IgG-F(ab')2 Fragment

Quantity:
0.5 mL

Primary or Secondary:
Secondary

Target Species:
Mouse

Isotype:
IgG

Applications:
Western Blot, ELISA, Immunohistochemistry, Immunofluorescence

Concentration:
0.5 mg/mL

Dilution:
Western Blot 1:2500-1:25000, ELISA 1:2500-1:25000, Immunohistochemistry 1:100-1:1000, Immunocytochemistry/Immunofluorescence 1:100-1:1000

Host Species:
Goat

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Test Specificity:
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to alkaline phosphatase. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.

Content And Storage:
Store at 4°C in the dark.

Img

Novus Biologicals

NB008597

--


Antigen:
IgG F(ab)2

Classification:
Polyclonal

Conjugate:
Biotin

Formulation:
Phosphate Buffered Saline (PBS) containing 0.2% BSA.

Immunogen:
Mouse IgG-F(ab')2 Fragment

Quantity:
0.5 mL

Primary or Secondary:
Secondary

Target Species:
Mouse

Isotype:
IgG

Applications:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry

Concentration:
0.5 mg/mL

Dilution:
Western Blot 1:10000 - 1:200000, ELISA 1:10000 - 1:200000, Immunohistochemistry 1:250 - 1:2500, Immunocytochemistry 1:100 - 1:500

Host Species:
Goat

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Test Specificity:
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to biotin. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.

Content And Storage:
Store at 4°C. Do not freeze.

Img

Novus Biologicals

NB008601

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Antigen:
IgG F(ab)2

Classification:
Polyclonal

Conjugate:
DyLight 488

Formulation:
Phosphate Buffered Saline (PBS) containing 0.2% BSA.

Immunogen:
Mouse IgG-F(ab')2 Fragment

Quantity:
0.5 mL

Primary or Secondary:
Secondary

Target Species:
Mouse

Isotype:
IgG

Applications:
Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Concentration:
0.5 mg/mL

Dilution:
Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunofluorescence 1:50 - 1:500, Immunocytochemistry 1:50 - 1:500

Host Species:
Goat

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Test Specificity:
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 488. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.

Content And Storage:
Store at 4°C. Do not freeze.

Img

Novus Biologicals

NB008602

--


Antigen:
IgG F(ab)2

Classification:
Polyclonal

Conjugate:
HRP

Formulation:
Phosphate Buffered Saline (PBS) containing 0.2% BSA.

Immunogen:
Mouse IgG-F(ab')2 Fragment

Quantity:
0.5 mL

Primary or Secondary:
Secondary

Target Species:
Mouse

Isotype:
IgG

Applications:
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry

Concentration:
0.5 mg/mL

Dilution:
Western Blot, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry 1:10-1:500

Host Species:
Goat

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Test Specificity:
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat F(ab')2 was detected. This antibody may cross react with F(ab')2 from other species.

Content And Storage:
Store at 4°C. Do not freeze.