SerpinB9 Antibody (7D8) - BSA Free, Novus Biologicals™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NB017568-Each-of-1 | In Stock | ₹ 17,668.14 |
NB017568 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,668.14
GST (18%): ₹ 3,180.265
Total Price: ₹ 20,848.405
Antigen
SerpinB9
Classification
Monoclonal
Concentration
1.0 mg/mL
Dilution
Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunohistochemistry-Frozen 1:10-1:500
Gene Accession No.
P50453
Gene Symbols
SERPINB9
Immunogen
Recombinant PI-9 produced in P. pastoris.
Quantity
0.025 mg
Research Discipline
Apoptosis, Immunology
Gene ID (Entrez)
5272
Target Species
Mouse (Negative), Porcine (Negative), Human
Form
Purified
Applications
Immunohistochemistry (Frozen), Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Flow Cytometry
Clone
7D8
Conjugate
Unconjugated
Formulation
PBS with 0.05% Sodium Azide
Gene Alias
CAP-3, CAP3PI-9, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI9protease inhibitor 9 (ovalbumin type), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9, serpin B9, serpin peptidase inhibitor, clade B (ovalbumin), member 9, serpin peptidase inhibitor, clade B, member 9
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
NB100-64359 is specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone 7D8 detects a band of approximately 42kDa under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence (2).
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype
IgG1
Related Products
Description
- SerpinB9 Monoclonal specifically detects SerpinB9 in Human, Mouse (Negative), Porcine (Negative) samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.
Compare Similar Items
Show Difference
Antigen: SerpinB9
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunohistochemistry-Frozen 1:10-1:500
Gene Accession No.: P50453
Gene Symbols: SERPINB9
Immunogen: Recombinant PI-9 produced in P. pastoris.
Quantity: 0.025 mg
Research Discipline: Apoptosis, Immunology
Gene ID (Entrez): 5272
Target Species: Mouse (Negative), Porcine (Negative), Human
Form: Purified
Applications: Immunohistochemistry (Frozen), Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Flow Cytometry
Clone: 7D8
Conjugate: Unconjugated
Formulation: PBS with 0.05% Sodium Azide
Gene Alias: CAP-3, CAP3PI-9, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI9protease inhibitor 9 (ovalbumin type), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9, serpin B9, serpin peptidase inhibitor, clade B (ovalbumin), member 9, serpin peptidase inhibitor, clade B, member 9
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: NB100-64359 is specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone 7D8 detects a band of approximately 42kDa under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence (2).
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1
Antigen: CD19
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Flow Cytometry 1:50-1:200, Immunoprecipitation 1:10-1:500
Gene Accession No.: P15391
Gene Symbols: CD19
Immunogen: Human EB-4 Burkitt lymphoma cell line.
Quantity: 0.025 mg
Research Discipline: Cytokine Research, Immunology, Innate Immunity, Mesenchymal Stem Cell Markers, Signal Transduction, Stem Cell Markers
Gene ID (Entrez): 930
Target Species: Human
Form: Purified
Applications: Immunoprecipitation, Flow Cytometry
Clone: Bu12
Conjugate: Unconjugated
Formulation: Phosphate buffered saline with 0.09% Sodium Azide
Gene Alias: B4, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, CD19 antigen, CD19 molecule, CVID3, Differentiation antigen CD19, MGC12802, T-cell surface antigen Leu-12
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: NB100-64360 recognizes human CD19, a 95kD cell surface glycoprotein, which is expressed on cells of the B cell lineage and follicular dendritic cells but absent on plasma cells. CD19 is an important signal transduction molecule which is involved in the regulation of B lymphocyte development, activation and differentiation.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1
Antigen: FIV gp95
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, Functional
Gene Accession No.: __
Gene Symbols: __
Immunogen: Made to FIV gp95
Quantity: 0.025 mg
Research Discipline: __
Gene ID (Entrez): __
Target Species: Virus
Form: Purified
Applications: Western Blot, Flow Cytometry, Functional Assay
Clone: SU1-30
Conjugate: Unconjugated
Formulation: PBS (pH 7.2) with 0.09% Sodium Azide
Gene Alias: __
Host Species: Mouse
Purification Method: Protein A purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: NB100-64378 specifically recognizes the relatively conserved E60 peptide (amino acid sequence SSWKQRNRWEWRPDF) located within the V3 region of FIV (Feline Immunodeficiency Virus) envelope glycoprotein SU,gp95. The primary receptor for FIV infection of cats has been identified as the T-cell activation antigen CD134 which, along with CXC chemokine receptor 4 (CXCR4), facilitates the infection of CD4+ primary target cells, resulting in a depletion in the CD4+ population. Studies have shown that the V3 loop of SU,gp95 is a major immunogenic domain, suggested to contain the main determinants for the binding of FIV SU,gp95 to CXCR4.(3) Clone SU1-30 has been shown to inhibit the sCD134-activated entry of both clade B and clade C FIV strains.(1) NB100-64378 does not recognize Feline Leukaemia Virus, Feline Herpes Virus type 1, Feline Coronavirus or Feline Calicivirus. Removal of Sodium Azide is recommended prior to use in functional studies.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1