NB200598F

RNA Polymerase II/POLR2A Antibody (4H8), FITC, Novus Biologicals™

Manufacturer: Novus Biologicals

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Pack Size SKU Availability Price
Each of 1 NB200598F-Each-of-1 In Stock ₹ 54,245.04

NB200598F - Each of 1

₹ 54,245.04

In Stock

Quantity

1

Base Price: ₹ 54,245.04

GST (18%): ₹ 9,764.107

Total Price: ₹ 64,009.147

Antigen

RNA Polymerase II/POLR2A

Classification

Monoclonal

Conjugate

FITC

Gene Accession No.

P24928

Gene Symbols

POLR2A

Immunogen

10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]

Purification Method

Protein G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recogn

Content And Storage

Store at 4C in the dark.

Isotype

IgG1

Applications

Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence

Clone

4H8

Dilution

Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)

Gene Alias

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2

Host Species

Mouse

Molecular Weight of Antigen

217 kDa

Quantity

0.1 mL

Research Discipline

Chromatin Research, Epigenetics, microRNA, Phospho Specific, Transcription Factors and Regulators

Gene ID (Entrez)

5430

Target Species

Human, Mouse, Yeast, Primate (Negative)

Form

Purified

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Description

  • RNA Polymerase II/POLR2A Monoclonal specifically detects RNA Polymerase II/POLR2A in Human, Mouse, Yeast, Primate (Negative) samples
  • It is validated for Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular).

Compare Similar Items

Show Difference

Img

Novus Biologicals

NB200598F

--


Antigen:
RNA Polymerase II/POLR2A

Classification:
Monoclonal

Conjugate:
FITC

Gene Accession No.:
P24928

Gene Symbols:
POLR2A

Immunogen:
10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recogn

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1

Applications:
Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence

Clone:
4H8

Dilution:
Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)

Gene Alias:
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2

Host Species:
Mouse

Molecular Weight of Antigen:
217 kDa

Quantity:
0.1 mL

Research Discipline:
Chromatin Research, Epigenetics, microRNA, Phospho Specific, Transcription Factors and Regulators

Gene ID (Entrez):
5430

Target Species:
Human, Mouse, Yeast, Primate (Negative)

Form:
Purified

Img

Novus Biologicals

NB200598FR

--


Antigen:
RNA Polymerase II/POLR2A

Classification:
Monoclonal

Conjugate:
DyLight 680

Gene Accession No.:
P24928

Gene Symbols:
POLR2A

Immunogen:
10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recogn

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1

Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence

Clone:
4H8

Dilution:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)

Gene Alias:
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2

Host Species:
Mouse

Molecular Weight of Antigen:
217 kDa

Quantity:
0.1 mL

Research Discipline:
Chromatin Research, Epigenetics, microRNA, Phospho Specific, Transcription Factors and Regulators

Gene ID (Entrez):
5430

Target Species:
Human, Mouse, Yeast, Primate (Negative)

Form:
Purified

Img

Novus Biologicals

NB200598G

--


Antigen:
RNA Polymerase II/POLR2A

Classification:
Monoclonal

Conjugate:
DyLight 488

Gene Accession No.:
P24928

Gene Symbols:
POLR2A

Immunogen:
10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recogn

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1

Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence

Clone:
4H8

Dilution:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)

Gene Alias:
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2

Host Species:
Mouse

Molecular Weight of Antigen:
217 kDa

Quantity:
0.1 mL

Research Discipline:
Chromatin Research, Epigenetics, microRNA, Phospho Specific, Transcription Factors and Regulators

Gene ID (Entrez):
5430

Target Species:
Human, Mouse, Yeast, Primate (Negative)

Form:
Purified

Img

Novus Biologicals

NB200598PE

--


Antigen:
RNA Polymerase II/POLR2A

Classification:
Monoclonal

Conjugate:
PE

Gene Accession No.:
P24928

Gene Symbols:
POLR2A

Immunogen:
10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]

Purification Method:
Protein G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recogn

Content And Storage:
Store at 4C in the dark.

Isotype:
IgG1

Applications:
Flow Cytometry

Clone:
4H8

Dilution:
Flow Cytometry, Flow (Intracellular)

Gene Alias:
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2

Host Species:
Mouse

Molecular Weight of Antigen:
217 kDa

Quantity:
0.1 mL

Research Discipline:
Chromatin Research, Epigenetics, microRNA, Phospho Specific, Transcription Factors and Regulators

Gene ID (Entrez):
5430

Target Species:
Human, Mouse, Yeast, Primate (Negative)

Form:
Purified