Manufacturer: Novus Biologicals
Pack Size | SKU | Availability | Price |
---|---|---|---|
Each of 1 | NB200598X-Each-of-1 | In Stock | ₹ 54,245.04 |
NB200598X - Each of 1
In Stock
Quantity
1
Base Price: ₹ 54,245.04
GST (18%): ₹ 9,764.107
Total Price: ₹ 64,009.147
RNA Polymerase II/POLR2A
Monoclonal
Alexa Fluor 488
P24928
POLR2A
10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProt# P24928]
Protein G purified
RUO
Primary
Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as 'hyper-' and 'hypo-phosphorylated', respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (though less likely) recogn
Store at 4C in the dark.
IgG1
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
4H8
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2
Mouse
217 kDa
0.1 mL
Chromatin Research, Epigenetics, microRNA, Phospho Specific, Transcription Factors and Regulators
5430
Human, Mouse, Yeast, Primate (Negative)
Purified