RNA Polymerase II/POLR2A Antibody (4H8), Alexa Fluor™ 350, Novus Biologicals™
Manufacturer: Novus Biologicals
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NB20598A350-Each-of-1 | In Stock | ₹ 54,245.04 |
NB20598A350 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 54,245.04
GST (18%): ₹ 9,764.107
Total Price: ₹ 64,009.147
Antigen
RNA Polymerase II/POLR2A
Classification
Monoclonal
Conjugate
Alexa Fluor 350
Formulation
50 mM sodium borate with 0.05% sodium azide
Gene Symbols
POLR2A
Immunogen
10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProtNo. P24928]
Quantity
0.1 mL
Primary or Secondary
Primary
Test Specificity
Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as ′hyper-′ and ′hypo-phosphorylated′, respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (th
Content And Storage
Store at 4°C in the dark.
Applications
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Clone
4H8
Dilution
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)
Gene Alias
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2
Host Species
Mouse
Purification Method
Protein G purified
Research Discipline
Chromatin Research, Epigenetics, microRNA, Phospho Specific, Transcription Factors and Regulators
Gene ID (Entrez)
5430.0
Target Species
Human, Mouse, Yeast, Primate
Isotype
IgG1
Related Products
Description
- RNA Polymerase II/POLR2A Monoclonal specifically detects RNA Polymerase II/POLR2A in Human, Mouse, Yeast, Primate (Negative) samples
- It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular).
Compare Similar Items
Show Difference
Antigen: RNA Polymerase II/POLR2A
Classification: Monoclonal
Conjugate: Alexa Fluor 350
Formulation: 50 mM sodium borate with 0.05% sodium azide
Gene Symbols: POLR2A
Immunogen: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProtNo. P24928]
Quantity: 0.1 mL
Primary or Secondary: Primary
Test Specificity: Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as ′hyper-′ and ′hypo-phosphorylated′, respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (th
Content And Storage: Store at 4°C in the dark.
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Clone: 4H8
Dilution: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)
Gene Alias: DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2
Host Species: Mouse
Purification Method: Protein G purified
Research Discipline: Chromatin Research, Epigenetics, microRNA, Phospho Specific, Transcription Factors and Regulators
Gene ID (Entrez): 5430.0
Target Species: Human, Mouse, Yeast, Primate
Isotype: IgG1
Antigen: RNA Polymerase II/POLR2A
Classification: Monoclonal
Conjugate: Alexa Fluor 532
Formulation: 50 mM sodium borate with 0.05% sodium azide
Gene Symbols: POLR2A
Immunogen: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProtNo. P24928]
Quantity: 0.1 mL
Primary or Secondary: Primary
Test Specificity: Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as ′hyper-′ and ′hypo-phosphorylated′, respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (th
Content And Storage: Store at 4°C in the dark.
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Clone: 4H8
Dilution: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)
Gene Alias: DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2
Host Species: Mouse
Purification Method: __
Research Discipline: __
Gene ID (Entrez): 5430
Target Species: Human, Mouse, Yeast, Primate
Isotype: IgG1
Antigen: RNA Polymerase II/POLR2A
Classification: Monoclonal
Conjugate: Alexa Fluor 594
Formulation: 50 mM sodium borate with 0.05% sodium azide
Gene Symbols: POLR2A
Immunogen: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProtNo. P24928]
Quantity: 0.1 mL
Primary or Secondary: Primary
Test Specificity: Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as ′hyper-′ and ′hypo-phosphorylated′, respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (th
Content And Storage: Store at 4°C in the dark.
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Clone: 4H8
Dilution: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)
Gene Alias: DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2
Host Species: Mouse
Purification Method: __
Research Discipline: __
Gene ID (Entrez): 5430
Target Species: Human, Mouse, Yeast, Primate
Isotype: IgG1