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NB20598A594

RNA Polymerase II/POLR2A Antibody (4H8), Alexa Fluor™ 594, Novus Biologicals™

Manufacturer: Novus Biologicals

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Pack Size SKU Availability Price
Each of 1 NB20598A594-Each-of-1 In Stock ₹ 54,245.04

NB20598A594 - Each of 1

₹ 54,245.04

In Stock

Quantity

1

Base Price: ₹ 54,245.04

GST (18%): ₹ 9,764.107

Total Price: ₹ 64,009.147

Antigen

RNA Polymerase II/POLR2A

Classification

Monoclonal

Conjugate

Alexa Fluor 594

Formulation

50 mM sodium borate with 0.05% sodium azide

Gene Symbols

POLR2A

Immunogen

10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProtNo. P24928]

Primary or Secondary

Primary

Test Specificity

Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as ′hyper-′ and ′hypo-phosphorylated′, respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (th

Content And Storage

Store at 4°C in the dark.

Applications

Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence

Clone

4H8

Dilution

Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)

Gene Alias

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2

Host Species

Mouse

Quantity

0.1 mL

Gene ID (Entrez)

5430

Target Species

Human, Mouse, Yeast, Primate

Isotype

IgG1

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Description

  • RNA Polymerase II/POLR2A Monoclonal specifically detects RNA Polymerase II/POLR2A in Human, Mouse, Yeast, Primate (Negative) samples
  • It is validated for Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular).

Compare Similar Items

Show Difference

Img

Novus Biologicals

NB20598A594

--

Antigen:
RNA Polymerase II/POLR2A
Classification:
Monoclonal
Conjugate:
Alexa Fluor 594
Formulation:
50 mM sodium borate with 0.05% sodium azide
Gene Symbols:
POLR2A
Immunogen:
10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). [UniProtNo. P24928]
Primary or Secondary:
Primary
Test Specificity:
Please note that this antibody detects both unphosphorylated and phosphorylated forms of the protein. When using this antibody in western blot and IP, it recognizes both the II0 and IIA form of RPB1 (ie the largest RNAPII subunit). These different forms of RPB1 (as seen in gels) have traditionally been loosely defined as ′hyper-′ and ′hypo-phosphorylated′, respectively. Here, however, it is important to remember that RPB1 contains no less than 52 repeats of a heptapeptide (TSPTSPS) that can be phosphorylated at different positions (Tyr1, Ser2, Thr4, Ser5 and Ser7; this antibody was raised against a synthetic peptide with 10 of these repeats, mostly but probably not entirely, chemically phosphorylated at Ser5). So, when we say that it recognizes both forms (II0/hyper-phos and IIA/hypo-phos), it might really just mean that the antibody recognizes a few phosphorylated Ser5s among 52 otherwise mostly un-phosphorylated repeats. Alternatively, it might conceivably (th
Content And Storage:
Store at 4°C in the dark.
Applications:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence
Clone:
4H8
Dilution:
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Flow (Intracellular)
Gene Alias:
DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kdsubunit, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, EC 2.7.7.48, EC 2.7.7.6, hRPB220, hsRPB1, MGC75453, POLR2, POLRA, polymerase (RNA) II (DNA directed) polypeptide A (220kD), polymerase (RNA) II (DNA directed) polypeptide A, 220kDa, RNA Pol 2, RNA Polymerase 2, RNA polymerase II subunit B1, RNA-directed RNA polymerase II subunit RPB1, RNAPII, RPB1, RPBh1, RpIILS, RPO2, RPOL2
Host Species:
Mouse
Quantity:
0.1 mL
Gene ID (Entrez):
5430
Target Species:
Human, Mouse, Yeast, Primate
Isotype:
IgG1

Img

Novus Biologicals

NB205990

--

Antigen:
NLRP5
Classification:
Polyclonal
Conjugate:
HRP
Formulation:
PBS
Gene Symbols:
__
Immunogen:
Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human NALP5. The immunogen is located within the last 50 amino acids of NALP5. Amino Acid Squence: WHSFDEDDRYWWKN
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Store at 4°C in the dark.
Applications:
Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Clone:
__
Dilution:
__
Gene Alias:
CLR19.8, MATERMater protein homolog, NACHT, leucine rich repeat and PYD containing 5, NACHT, LRR and PYD domains-containing protein 5, NALP5maternal antigen that embryos require, NLR family, pyrin domain containing 5, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 5, PAN11, PYPAF8
Host Species:
Rabbit
Quantity:
0.1 mL
Gene ID (Entrez):
126206
Target Species:
Human, Mouse, Rat
Isotype:
IgG

Img

Novus Biologicals

NB205991

--

Antigen:
RHBDD2
Classification:
Polyclonal
Conjugate:
FITC
Formulation:
PBS
Gene Symbols:
__
Immunogen:
Antibody was raised against an 18 amino acid synthetic peptide from near the center of human RHBDD2. The immunogen is located within amino acids 220 - 270 of RHBDD2. Amino Acid Squence: AERRAAQSRKLMPVPGSY
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Store at 4°C in the dark.
Applications:
ELISA, Immunofluorescence
Clone:
__
Dilution:
__
Gene Alias:
H_RG122E10.2a, H_RG122E10.2b, rhomboid domain containing 2, rhomboid domain-containing protein 2, rhomboid, veinlet-like 7, veinlet-like 7 (Drosophila)
Host Species:
Rabbit
Quantity:
0.1 mL
Gene ID (Entrez):
57414
Target Species:
Human, Mouse, Rat
Isotype:
IgG

Img

Novus Biologicals

NB205996

--

Antigen:
RHBDD2
Classification:
Polyclonal
Conjugate:
DyLight 680
Formulation:
50mM Sodium Borate
Gene Symbols:
__
Immunogen:
Antibody was raised against an 18 amino acid synthetic peptide from near the center of human RHBDD2. The immunogen is located within amino acids 220 - 270 of RHBDD2. Amino Acid Squence: AERRAAQSRKLMPVPGSY
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Store at 4°C in the dark.
Applications:
Western Blot, ELISA, Immunofluorescence
Clone:
__
Dilution:
__
Gene Alias:
H_RG122E10.2a, H_RG122E10.2b, rhomboid domain containing 2, rhomboid domain-containing protein 2, rhomboid, veinlet-like 7, veinlet-like 7 (Drosophila)
Host Species:
Rabbit
Quantity:
0.1 mL
Gene ID (Entrez):
57414
Target Species:
Human, Mouse, Rat
Isotype:
IgG