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NB254452200

Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free, Novus Biologicals™

Manufacturer: Fischer Scientific

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Antigen

Enolase 2/Neuron-specific Enolase

Classification

Monoclonal

Concentration

1 mg/mL

Dilution

Western Blot 0.5 - 1 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells, Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml, Immunohistochemistry-Paraffin 0.1 - 0.2 ug/ml, CyTOF-ready

Gene Alias

2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11, Enolase 2, enolase 2 (gamma, neuronal), gamma-enolase, Neural enolase, neuron specific gamma enolase, neurone-specific enolase, Neuron-specific enolase, NSE

Host Species

Mouse

Molecular Weight of Antigen

50 kDa

Quantity

200 μg

Research Discipline

Cancer, Cellular Markers, Neuronal Cell Markers, Neuroscience

Gene ID (Entrez)

2026

Target Species

Human, Mouse, Rat

Form

Purified

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), CyTOF

Clone

ENO2/1375

Conjugate

Unconjugated

Formulation

PBS with No Preservative

Gene Symbols

ENO2

Immunogen

A synthetic peptide corresponding to aa416-433 of human NSE gamma.

Purification Method

Protein A or G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Content And Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Isotype

IgG

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Description

  • Enolase 2/Neuron-specific Enolase Monoclonal specifically detects Enolase 2/Neuron-specific Enolase in Human, Mouse, Rat samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Flow (Intracellular), CyTOF-ready.

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Fischer Scientific

NB254452200

--

Antigen:
Enolase 2/Neuron-specific Enolase
Classification:
Monoclonal
Concentration:
1 mg/mL
Dilution:
Western Blot 0.5 - 1 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells, Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml, Immunohistochemistry-Paraffin 0.1 - 0.2 ug/ml, CyTOF-ready
Gene Alias:
2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, EC 4.2.1.11, Enolase 2, enolase 2 (gamma, neuronal), gamma-enolase, Neural enolase, neuron specific gamma enolase, neurone-specific enolase, Neuron-specific enolase, NSE
Host Species:
Mouse
Molecular Weight of Antigen:
50 kDa
Quantity:
200 μg
Research Discipline:
Cancer, Cellular Markers, Neuronal Cell Markers, Neuroscience
Gene ID (Entrez):
2026
Target Species:
Human, Mouse, Rat
Form:
Purified
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), CyTOF
Clone:
ENO2/1375
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
ENO2
Immunogen:
A synthetic peptide corresponding to aa416-433 of human NSE gamma.
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Content And Storage:
Store at -20 to -80C. Avoid freeze-thaw cycles.
Isotype:
IgG

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NB254453100

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Antigen:
Cytokeratin 15
Classification:
Monoclonal
Concentration:
1 mg/mL
Dilution:
Western Blot 0.5 - 1 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells, Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias:
CK15, CK-15, cytokeratin 15, cytokeratin-15, K15keratin-15, basic, K1CO, keratin 15, keratin, type I cytoskeletal 15, Keratin-15, keratin-15, beta, KRTB, type I cytoskeletal 15
Host Species:
Mouse
Molecular Weight of Antigen:
52 kDa
Quantity:
100 μg
Research Discipline:
Cytoskeleton Markers
Gene ID (Entrez):
3866
Target Species:
Human, Rat, Bovine
Form:
Purified
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Clone:
SPM190
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
KRT15
Immunogen:
A 17 amino acids synthetic peptide from the C- terminal of Human Cytokeratin 15
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Store at -20 to -80C. Avoid freeze-thaw cycles.
Isotype:
IgG2a κ

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NB254453200

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Antigen:
Cytokeratin 15
Classification:
Monoclonal
Concentration:
1 mg/mL
Dilution:
Western Blot 0.5 - 1 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells, Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias:
CK15, CK-15, cytokeratin 15, cytokeratin-15, K15keratin-15, basic, K1CO, keratin 15, keratin, type I cytoskeletal 15, Keratin-15, keratin-15, beta, KRTB, type I cytoskeletal 15
Host Species:
Mouse
Molecular Weight of Antigen:
52 kDa
Quantity:
200 μg
Research Discipline:
Cytoskeleton Markers
Gene ID (Entrez):
3866
Target Species:
Human, Rat, Bovine
Form:
Purified
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Clone:
SPM190
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
KRT15
Immunogen:
A 17 amino acids synthetic peptide from the C- terminal of Human Cytokeratin 15
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Store at -20 to -80C. Avoid freeze-thaw cycles.
Isotype:
IgG2a κ

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NB254454100

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Antigen:
PMEL17/SILV
Classification:
Monoclonal
Concentration:
1 mg/mL
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells, Immunocytochemistry/Immunofluorescence 0.5 - 1 ug/ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias:
D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Host Species:
Rabbit
Molecular Weight of Antigen:
95 kDa
Quantity:
100 μg
Research Discipline:
__
Gene ID (Entrez):
6490
Target Species:
Human
Form:
Purified
Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), CyTOF
Clone:
PMEL/1825R
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
PMEL
Immunogen:
Recombinant full-length human SILV protein
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
Store at -20 to -80C. Avoid freeze-thaw cycles.
Isotype:
IgG κ