NBP142270AG
Human IgA Antibody (BFG-267), Alexa Fluor™ 488, Novus Biologicals™
Manufacturer: Novus Biologicals
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NBP142270AG-Each-of-1 | In Stock | ₹ 48,769.20 |
NBP142270AG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 48,769.20
GST (18%): ₹ 8,778.456
Total Price: ₹ 57,547.656
Antigen
Human IgA
Classification
Monoclonal
Conjugate
Alexa Fluor 488
Formulation
50mM Sodium Borate with 0.05% Sodium Azide
Immunogen
Purified alpha-chain (Fc) of human IgA
Quantity
0.2 mL
Primary or Secondary
Primary
Target Species
Human
Form
Purified
Applications
ELISA
Clone
BFG-267
Dilution
ELISA
Host Species
Mouse
Purification Method
Ion exchange chromatography
Regulatory Status
RUO
Test Specificity
This monoclonal antibody reacts with human IgA, alpha-chain. No cross reaction with human plasma proteins other then human IgA. No cross reaction with purified human IgE, IgG and IgM. Apparent binding constant is approximately 0.01 nM. Conjugation of the antibody with peroxidase, FITC or biotin does not impair the binding properties. Specificity of the monoclonal was determined by ELISA on purified human immunoglobulin isotypes.
Content And Storage
Store at 4C in the dark.
Isotype
IgG1
Description
- Human IgA Monoclonal specifically detects Human IgA in Human samples
- It is validated for ELISA.
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Antigen: Human IgA
Classification: Monoclonal
Conjugate: Alexa Fluor 488
Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
Immunogen: Purified alpha-chain (Fc) of human IgA
Quantity: 0.2 mL
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Applications: ELISA
Clone: BFG-267
Dilution: ELISA
Host Species: Mouse
Purification Method: Ion exchange chromatography
Regulatory Status: RUO
Test Specificity: This monoclonal antibody reacts with human IgA, alpha-chain. No cross reaction with human plasma proteins other then human IgA. No cross reaction with purified human IgE, IgG and IgM. Apparent binding constant is approximately 0.01 nM. Conjugation of the antibody with peroxidase, FITC or biotin does not impair the binding properties. Specificity of the monoclonal was determined by ELISA on purified human immunoglobulin isotypes.
Content And Storage: Store at 4C in the dark.
Isotype: IgG1
Antigen:
Human IgA
Classification:
Monoclonal
Conjugate:
Alexa Fluor 488
Formulation:
50mM Sodium Borate with 0.05% Sodium Azide
Immunogen:
Purified alpha-chain (Fc) of human IgA
Quantity:
0.2 mL
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Applications:
ELISA
Clone:
BFG-267
Dilution:
ELISA
Host Species:
Mouse
Purification Method:
Ion exchange chromatography
Regulatory Status:
RUO
Test Specificity:
This monoclonal antibody reacts with human IgA, alpha-chain. No cross reaction with human plasma proteins other then human IgA. No cross reaction with purified human IgE, IgG and IgM. Apparent binding constant is approximately 0.01 nM. Conjugation of the antibody with peroxidase, FITC or biotin does not impair the binding properties. Specificity of the monoclonal was determined by ELISA on purified human immunoglobulin isotypes.
Content And Storage:
Store at 4C in the dark.
Isotype:
IgG1
Antigen: Tenascin C
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: __
Immunogen: The antibody reacts with the fibrinogen-like knob-domain of tenascin protein.
Quantity: 0.05 mg
Primary or Secondary: Primary
Target Species: Human, Rat
Form: Purified
Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone: DB7
Dilution: Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Test Specificity: The antibody recognizes the Mr 250000 and 180000 human tenascin polypeptides in immunoprecipitation and in immunoblots the Mr 250000 polypeptide. The antibody reacts with the fibrinogen-like knob-domain of tenascin protein. The antibody reacts especially with human paraffin sections. Tenascins were first characterized in the early 1980's. Since then hundreds of publications on tenascin in normal tissues, pathologically reactive tissues and carcinomas have been published. However, only recently more elective studies have been done. In these studies retrospective material from pathology files has been studied as well as larger fresh material collected during several years from patients. These studies have now attempted to reveal more specific points in carcinogenesis and pathological tissue reactions. It has been demonstrated that tenascin immunoreactivity in breast carcinoma cells could be indicative of metastasis and survival. Recent studies using retrospective material showed that the expression of tenascin in invasion border of early breast cancer significantly correlates with higher risk of distant metastasis. These studies have been continued now and the preliminary results clearly suggest that expression of tenascin in invasion border of early breast cancer is significantly associated with proliferative activity and higher risk of local recurrence. This result implicates a wide application for tenascin antibodies in the breast pathology. The point is that tenascin expression may suggest situations in which even small breast carcinomas may need more intensive complementary therapy (chemotherapy etc.). Recent studies have also shown that tenascin is significantly increased in airway basement membrane of asthmatics and rapidly decreases by inhaled steroid. This result suggests that tenascin expression may be used to monitor the disease status and outcome of treatment in different types of asthma. The monoclonal antibody to tenascin (clone DB7) is suitable for immunohistochemistry with formalin-fixed and paraffin-embedded sections. The other monoclonal antibody (clone EB2) is highly sensitive in Western blotting. Monoclonal antibody (Mab) to tenascin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Tenascin polypeptides isolated from spent culture supernatant of human fibroblasts isolated by affinity chromatography were used as immunogen.
Content And Storage: Store at 4C. Do not freeze.
Isotype: IgG2a
Antigen:
Tenascin C
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
__
Immunogen:
The antibody reacts with the fibrinogen-like knob-domain of tenascin protein.
Quantity:
0.05 mg
Primary or Secondary:
Primary
Target Species:
Human, Rat
Form:
Purified
Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone:
DB7
Dilution:
Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Test Specificity:
The antibody recognizes the Mr 250000 and 180000 human tenascin polypeptides in immunoprecipitation and in immunoblots the Mr 250000 polypeptide. The antibody reacts with the fibrinogen-like knob-domain of tenascin protein. The antibody reacts especially with human paraffin sections. Tenascins were first characterized in the early 1980's. Since then hundreds of publications on tenascin in normal tissues, pathologically reactive tissues and carcinomas have been published. However, only recently more elective studies have been done. In these studies retrospective material from pathology files has been studied as well as larger fresh material collected during several years from patients. These studies have now attempted to reveal more specific points in carcinogenesis and pathological tissue reactions. It has been demonstrated that tenascin immunoreactivity in breast carcinoma cells could be indicative of metastasis and survival. Recent studies using retrospective material showed that the expression of tenascin in invasion border of early breast cancer significantly correlates with higher risk of distant metastasis. These studies have been continued now and the preliminary results clearly suggest that expression of tenascin in invasion border of early breast cancer is significantly associated with proliferative activity and higher risk of local recurrence. This result implicates a wide application for tenascin antibodies in the breast pathology. The point is that tenascin expression may suggest situations in which even small breast carcinomas may need more intensive complementary therapy (chemotherapy etc.). Recent studies have also shown that tenascin is significantly increased in airway basement membrane of asthmatics and rapidly decreases by inhaled steroid. This result suggests that tenascin expression may be used to monitor the disease status and outcome of treatment in different types of asthma. The monoclonal antibody to tenascin (clone DB7) is suitable for immunohistochemistry with formalin-fixed and paraffin-embedded sections. The other monoclonal antibody (clone EB2) is highly sensitive in Western blotting. Monoclonal antibody (Mab) to tenascin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Tenascin polypeptides isolated from spent culture supernatant of human fibroblasts isolated by affinity chromatography were used as immunogen.
Content And Storage:
Store at 4C. Do not freeze.
Isotype:
IgG2a
Antigen: Alpha Fodrin
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: __
Immunogen: The antibody reacts with the alpha-Fodrin antigen.
Quantity: 0.05 mg
Primary or Secondary: Primary
Target Species: Human, Rat
Form: Purified
Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Frozen)
Clone: AA6
Dilution: Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Test Specificity: The antibody is specific to the 240 kD a-fodrin molecule of all mammalian nonerythroid cells and chicken a-spectrin. The antibody cross reacts with human, rat, chicken, rabbit and guinea pig. Monoclonal antibody to a-fodrin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation were used as immunogen.
Content And Storage: Store at 4C. Do not freeze.
Isotype: IgG1
Antigen:
Alpha Fodrin
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
__
Immunogen:
The antibody reacts with the alpha-Fodrin antigen.
Quantity:
0.05 mg
Primary or Secondary:
Primary
Target Species:
Human, Rat
Form:
Purified
Applications:
Western Blot, Immunohistochemistry, Immunohistochemistry (Frozen)
Clone:
AA6
Dilution:
Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Test Specificity:
The antibody is specific to the 240 kD a-fodrin molecule of all mammalian nonerythroid cells and chicken a-spectrin. The antibody cross reacts with human, rat, chicken, rabbit and guinea pig. Monoclonal antibody to a-fodrin is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation were used as immunogen.
Content And Storage:
Store at 4C. Do not freeze.
Isotype:
IgG1
Antigen: Mouse IgM
Classification: Monoclonal
Conjugate: Alexa Fluor 488
Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
Immunogen: The antibody reacts with the Mouse IgM antigen.
Quantity: 0.2 mL
Primary or Secondary: Primary
Target Species: Mouse
Form: Purified
Applications: ELISA, Immunohistochemistry, Immunohistochemistry (Frozen)
Clone: LO-MM-9
Dilution: ELISA, Immunohistochemistry, Immunohistochemistry-Frozen
Host Species: Rat
Purification Method: Protein A or G purified
Regulatory Status: RUO
Test Specificity: Mouse mu heavy chain of immunoglobulin (determined by immunodot). Avidity on IgM: 7 * 10^8 M-1. No cross reactivity with other species.
Content And Storage: Store at 4C in the dark.
Isotype: IgG2a κ
Antigen:
Mouse IgM
Classification:
Monoclonal
Conjugate:
Alexa Fluor 488
Formulation:
50mM Sodium Borate with 0.05% Sodium Azide
Immunogen:
The antibody reacts with the Mouse IgM antigen.
Quantity:
0.2 mL
Primary or Secondary:
Primary
Target Species:
Mouse
Form:
Purified
Applications:
ELISA, Immunohistochemistry, Immunohistochemistry (Frozen)
Clone:
LO-MM-9
Dilution:
ELISA, Immunohistochemistry, Immunohistochemistry-Frozen
Host Species:
Rat
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Test Specificity:
Mouse mu heavy chain of immunoglobulin (determined by immunodot). Avidity on IgM: 7 * 10^8 M-1. No cross reactivity with other species.
Content And Storage:
Store at 4C in the dark.
Isotype:
IgG2a κ