Smad3, p Ser423, p Ser425 Antibody, Novus Biologicals™
Manufacturer: Novus Biologicals
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NBP177836SS-Each-of-1 | In Stock | ₹ 22,930.08 |
NBP177836SS - Each of 1
In Stock
Quantity
1
Base Price: ₹ 22,930.08
GST (18%): ₹ 4,127.414
Total Price: ₹ 27,057.494
Antigen
Smad3 (p Ser423, p Ser425)
Classification
Polyclonal
Conjugate
Unconjugated
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Gene Alias
DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), Mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, pSMAD, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Host Species
Rabbit
Molecular Weight of Antigen
48.08 kDa
Quantity
0.025 mg
Primary or Secondary
Primary
Test Specificity
This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Content And Storage
Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Applications
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Concentration
1.1 mg/ml
Dilution
Western Blot 1:2000-1:20000, Flow Cytometry 1:10-1:1000, ELISA 1:15000-1:30000, Immunohistochemistry 1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:10-1:500, Single Cell Western 100 ug/ml
Gene Accession No.
NC_000015.9
Gene Symbols
SMAD3
Immunogen
Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)
Purification Method
Affinity Purified
Regulatory Status
RUO
Gene ID (Entrez)
4088
Target Species
Human, Mouse, Rat, Porcine, Bovine, Chicken, Xenopus species, Zebrafish
Isotype
IgG
Related Products
Description
- Smad3 (p Ser423, p Ser425) Polyclonal antibody specifically detects Smad3 (p Ser423, p Ser425) in Human, Mouse samples
- It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Single Cell Western.
Compare Similar Items
Show Difference
Antigen: Smad3 (p Ser423, p Ser425)
Classification: Polyclonal
Conjugate: Unconjugated
Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Gene Alias: DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), Mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, pSMAD, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Host Species: Rabbit
Molecular Weight of Antigen: 48.08 kDa
Quantity: 0.025 mg
Primary or Secondary: Primary
Test Specificity: This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Concentration: 1.1 mg/ml
Dilution: Western Blot 1:2000-1:20000, Flow Cytometry 1:10-1:1000, ELISA 1:15000-1:30000, Immunohistochemistry 1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:10-1:500, Single Cell Western 100 ug/ml
Gene Accession No.: NC_000015.9
Gene Symbols: SMAD3
Immunogen: Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)
Purification Method: Affinity Purified
Regulatory Status: RUO
Gene ID (Entrez): 4088
Target Species: Human, Mouse, Rat, Porcine, Bovine, Chicken, Xenopus species, Zebrafish
Isotype: IgG
Antigen: Smad3 (p Ser423)
Classification: Polyclonal
Conjugate: DyLight 350
Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
Gene Alias: DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Host Species: Rabbit
Molecular Weight of Antigen: __
Quantity: 0.1 mL
Primary or Secondary: Primary
Test Specificity: This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Content And Storage: Store at 4C in the dark.
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Concentration: __
Dilution: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin
Gene Accession No.: __
Gene Symbols: SMAD3
Immunogen: Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)
Purification Method: Affinity Purified
Regulatory Status: __
Gene ID (Entrez): 4088
Target Species: Human, Mouse, Rat, Porcine, Bovine, Chicken, Xenopus species, Zebrafish
Isotype: IgG
Antigen: KAT4/TBP Associated Factor 1
Classification: Polyclonal
Conjugate: Unconjugated
Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Gene Alias: 250kDa, BA2Rdystonia 3 (with Parkinsonism), CCG1TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 250kD, CCGS, Cell cycle gene 1 protein, cell cycle, G1 phase defect, complementation of cell cycle block, G1-to-S, DYT3/TAF1, EC 2.7.11, EC 2.7.11.1, KAT4, NSCL2, OF, P250, TAF(II)250, TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, TAF2AN-TAF1, TAFII-250, TAFII250DYT3, TBP-associated factor 250 kDa, transcription factor TFIID p250 polypeptide, Transcription initiation factor TFIID 250 kDa subunit, transcription initiation factor TFIID subunit 1, XDP
Host Species: Rabbit
Molecular Weight of Antigen: 212.68 kDa
Quantity: 0.1 mg
Primary or Secondary: Primary
Test Specificity: A BLAST analysis was used to suggest cross-reactivity with TAF1 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin)
Concentration: 1.1 mg/mL
Dilution: Western Blot 1:100-1:2000, ELISA 1:150000-1:300000, Immunohistochemistry 10ug/ml, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 10 ug/ml, Chromatin Immunoprecipitation (ChIP) 1:10-1:500
Gene Accession No.: P21675
Gene Symbols: TAF1
Immunogen: This affinity purified KAT4/TBP Associated Factor 1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human KAT4/TBP Associated Factor 1. (Uniprot: P21675)
Purification Method: Affinity Purified
Regulatory Status: RUO
Gene ID (Entrez): 6872
Target Species: Human, Mouse, Rat
Isotype: IgG
