NBP177836UV

Smad3, p Ser423, p Ser425 Antibody, DyLight 350, Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NBP177836UV-Each-of-1 In Stock ₹ 67,763.52

NBP177836UV - Each of 1

₹ 67,763.52

In Stock

Quantity

1

Base Price: ₹ 67,763.52

GST (18%): ₹ 12,197.434

Total Price: ₹ 79,960.954

Antigen

Smad3 (p Ser423)

Classification

Polyclonal

Dilution

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin

Gene Alias

DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3

Host Species

Rabbit

Purification Method

Affinity Purified

Research Discipline

Phospho Specific, Signal Transduction, Wnt Signaling Pathway

Gene ID (Entrez)

4088

Target Species

Human, Mouse, Rat, Porcine, Bovine, Chicken, Xenopus species, Zebrafish

Isotype

IgG

Applications

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)

Conjugate

DyLight 350

Formulation

50mM Sodium Borate with 0.05% Sodium Azide

Gene Symbols

SMAD3

Immunogen

Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)

Quantity

0.1 mL

Primary or Secondary

Primary

Test Specificity

This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Content And Storage

Store at 4C in the dark.

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Description

  • Smad3 (p Ser423) Polyclonal antibody specifically detects Smad3 (p Ser423) in Human, Mouse samples
  • It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin).

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Show Difference

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Novus Biologicals

NBP177836UV

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Antigen:
Smad3 (p Ser423)

Classification:
Polyclonal

Dilution:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin

Gene Alias:
DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3

Host Species:
Rabbit

Purification Method:
Affinity Purified

Research Discipline:
Phospho Specific, Signal Transduction, Wnt Signaling Pathway

Gene ID (Entrez):
4088

Target Species:
Human, Mouse, Rat, Porcine, Bovine, Chicken, Xenopus species, Zebrafish

Isotype:
IgG

Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)

Conjugate:
DyLight 350

Formulation:
50mM Sodium Borate with 0.05% Sodium Azide

Gene Symbols:
SMAD3

Immunogen:
Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)

Quantity:
0.1 mL

Primary or Secondary:
Primary

Test Specificity:
This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Content And Storage:
Store at 4C in the dark.

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NBP177847

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Antigen:
KAT4/TBP Associated Factor 1

Classification:
Polyclonal

Dilution:
Western Blot 1:100-1:2000, ELISA 1:150000-1:300000, Immunohistochemistry 10ug/ml, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 10 ug/ml, Chromatin Immunoprecipitation (ChIP) 1:10-1:500

Gene Alias:
250kDa, BA2Rdystonia 3 (with Parkinsonism), CCG1TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 250kD, CCGS, Cell cycle gene 1 protein, cell cycle, G1 phase defect, complementation of cell cycle block, G1-to-S, DYT3/TAF1, EC 2.7.11, EC 2.7.11.1, KAT4, NSCL2, OF, P250, TAF(II)250, TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, TAF2AN-TAF1, TAFII-250, TAFII250DYT3, TBP-associated factor 250 kDa, transcription factor TFIID p250 polypeptide, Transcription initiation factor TFIID 250 kDa subunit, transcription initiation factor TFIID subunit 1, XDP

Host Species:
Rabbit

Purification Method:
Affinity Purified

Research Discipline:
Protein Kinase

Gene ID (Entrez):
6872

Target Species:
Human, Mouse, Rat

Isotype:
IgG

Applications:
Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide

Gene Symbols:
TAF1

Immunogen:
This affinity purified KAT4/TBP Associated Factor 1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human KAT4/TBP Associated Factor 1. (Uniprot: P21675)

Quantity:
0.1 mg

Primary or Secondary:
Primary

Test Specificity:
A BLAST analysis was used to suggest cross-reactivity with TAF1 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Content And Storage:
Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

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NBP177851

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Antigen:
STAT5a (p Tyr694)

Classification:
Polyclonal

Dilution:
Western Blot 1:500-1:2000, ELISA 1:25000, Immunohistochemistry 20-40 ug/ml, Immunohistochemistry-Paraffin 1:10-1:500

Gene Alias:
MGF, signal transducer and activator of transcription 5A, STAT5

Host Species:
Rabbit

Purification Method:
Affinity Purified

Research Discipline:
Cancer, Phospho Specific, Signal Transduction, Transcription Factors and Regulators

Gene ID (Entrez):
6776

Target Species:
Human, Mouse, Rat

Isotype:
IgG

Applications:
Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide

Gene Symbols:
STAT5A

Immunogen:
This affinity purified STAT5A [p Tyr694] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5A protein. (Uniprot: P42230)

Quantity:
0.1 mg

Primary or Secondary:
Primary

Test Specificity:
This antibody is specific for mouse STAT5A protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with STAT5A from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with STAT5A from other sources has not been determined.

Content And Storage:
Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

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NBP177858

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Antigen:
Smad1 (p Ser206)

Classification:
Polyclonal

Dilution:
Western Blot 1:500-1:2000, ELISA 1:5000-1:25000

Gene Alias:
BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, pSMAD, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1

Host Species:
Rabbit

Purification Method:
Affinity Purified

Research Discipline:
__

Gene ID (Entrez):
4086

Target Species:
Human, Mouse, Rat, Canine

Isotype:
IgG

Applications:
Western Blot, ELISA

Conjugate:
Unconjugated

Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide

Gene Symbols:
SMAD1

Immunogen:
This affinity purified Smad1 [p Ser206] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of human Smad1 protein. (Uniprot: Q15797)

Quantity:
0.1 mg

Primary or Secondary:
Primary

Test Specificity:
This affinity-purified antibody is directed against the phosphorylated form of human Smad1 protein at the pS206 residue. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad1 pS206 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest 100% cross reactivity with Smad1 from human, Duckbill platypus, Drosophila, mouse, opossum, chimpanzee, bonobo, orangutan, gorilla, macaque, cattle, sheep, pig, rat, horse, salmon, chicken, and dog based on the sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Content And Storage:
Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.