Antigen:
DYKDDDDK Epitope Tag
Classification:
Monoclonal
Purification Method:
Affinity Purified
Primary or Secondary:
Primary
Reconstitution:
Reconstitute with 100 ul deionized water (or equivalent)
Content And Storage:
Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Dilution:
Western Blot 1:2000-1:10000, Flow Cytometry, ELISA 1:100000, Immunohistochemistry 1:1000-1:5000
Gene Alias:
DDDDK epitope tag, DDK, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, epitope tag flag, FLAG (FLAG is a trademark of Sigma Aldrich), Flag tag, Ty 1 Tag, xxxDDDDK epitope tag
Immunogen:
This DYKDDDDK Epitope Tag Antibody (29E4.G7) was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG(TM) epitope tag peptide DYKDDDDK Epitope Tag Epitope Tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.
Test Specificity:
The purified antibody is directed against the FLAG(TM) motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins. This monoclonal anti-FLAG(TM) tag antibody detects over-expressed proteins containing the FLAG(TM) epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.